CHARACTERIZATION OF A CANDIDATE GENE FOR GP72, AN INSECT STAGE-SPECIFIC ANTIGEN OF TRYPANOSOMA-CRUZI

被引：35

作者：

COOPER, R

INVERSO, JA

ESPINOSA, M

NOGUEIRA, N

CROSS, GAM

机构：

[1] Laboratory of Molecular Parasitology, The Rockefeller University, New York, NY

来源：

MOLECULAR AND BIOCHEMICAL PARASITOLOGY | 1991年 / 49卷 / 01期

关键词：

TRYPANOSOMA-CRUZI; GLYCOPROTEIN; GENE; DIFFERENTIATION ANTIGEN; POLYMERASE CHAIN REACTION; PROTEIN SEQUENCE;

D O I：

10.1016/0166-6851(91)90129-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Trypanosoma cruzi insect stage-specific antigen GP72 was purified from epimastigotes and the amino acid sequences of peptide fragments determined. Oligonucleotides derived from these data were used to amplify and clone a cDNA sequence, which was used to isolate a full-length gene. All the sequenced peptides were encoded within the gene. The characteristics of the encoded 62 600-Da protein, including a potential amino-terminal signal sequence, a hydrophobic carboxy-terminus, and a large number of potential O-glycosylation sites, are consistent with the properties of GP72. No sequence homologies were found in searches of DNA and protein data banks. GP72 is encoded by a single pair of non-telomeric allelic genes.

引用

页码：45 / 60

页数：16

共 49 条

[1] INTERNAL AMINO-ACID SEQUENCE-ANALYSIS OF PROTEINS SEPARATED BY ONE-DIMENSIONAL OR TWO-DIMENSIONAL GEL-ELECTROPHORESIS AFTER INSITU PROTEASE DIGESTION ON NITROCELLULOSE [J].