ALTERED SYNTHESIS OF SOME SECRETORY PROTEINS IN PANCREATIC LOBULES ISOLATED FROM STREPTOZOTOCIN-INDUCED DIABETIC RATS

被引：9

作者：

DUAN, RD ^{[1
]}

ERLANSONALBERTSSON, C ^{[1
]}

机构：

[1] UNIV LUND,DEPT MED & PHYSIOL CHEM,POB 94,S-22100 LUND,SWEDEN

来源：

PANCREAS | 1990年 / 5卷 / 02期

关键词：

Amylase; Colipase; Diabetic rats; Enzyme synthesis; Lipase; Procarboxypeptidases; Serine proteases;

D O I：

10.1097/00006676-199003000-00003

中图分类号：

R57 [消化系及腹部疾病];

学科分类号：

摘要：

The in vitro incorporation of [35S]cysteine into lipase, colipase, amylase, procarboxypeptidase A and B, and the serine proteases and total proteins was studied in pancreatic lobules isolated from normal and diabetic rats with or without insulin treatment. The incorporation of [35S]cysteine into total proteins was 65% greater in pancreatic lobules from diabetic animals than from normal rats. The increased incorporation was partly reversed by insulin treatment (2 U/100 g/day for 5 days) of diabetic rats. The relative rates of biosynthesis for amylase and the procarboxypeptidases in diabetic pancreatic lobules were decreased by 75 and 25%, respectively, after 1 h of incubation, while those for lipase, colipase, and the serine proteases were increased by 90, 85, and 35%, respectively. The absolute rates of synthesis for these enzymes changed in the same direction as the relative rates in diabetic lobules, except that for the procarboxypeptidases, which did not change. The changed rates of biosynthesis for the pancreatic enzymes were reversed by insulin treatment of the diabetic rats. Kinetic studies showed that the incorporation of [35S]cysteine into amylase, lipase, and colipase was linear until up to 2 h of incubation in normal pancreatic lobules, while in the diabetic lobules the incorporation into lipase and colipase was accelerated, reaching a plateau level already after 1 h of incubation. It is concluded that the biosynthesis of pancreatic secretory proteins in diabetic rats is greatly changed both in terms of quantity and kinetics. © 1990 Raven Press, Ltd., New York.

引用

页码：136 / 143

页数：8

共 39 条

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