FAST PROTEIN CHROMATOGRAPHY ON ANALYTICAL AND PREPARATIVE SCALE USING MODIFIED MICROPOROUS MEMBRANES

Highly purified proteins and enzymes are usually obtained using chromatographic separations which require long cycle times. To improve mass transport other types of chromatographic support than porous particles seem to be interesting. In this paper a fixed bed composed of about 100 stacked microporous membranes is investigated for protein chromatography. A mathematical model and its experimental verification indicate the condition under which nonlinear, kinetic and dispersive effects will occur. A scale-up of two protein purifications and their incorporation in the complete recovery procedure is also presented. The two proteins are formate dehydrogenase (FDH) from Candida bodinii and pyruvate decarboxylase (PDC) from Zymomonas mobilis.