UNFOLDING OF THE TERTIARY STRUCTURE OF SPECIFIC TRANSFER-RNA AND RIBOSOMAL 5S RNA FROM PLANTS AS STUDIED WITH HYDROXYL RADICALS

被引：4

作者：

BARCISZEWSKA, MZ ^{[1
]}

BARCISZEWSKI, J ^{[1
]}

ERDMANN, VA ^{[1
]}

机构：

[1] FREE UNIV BERLIN,INST BIOCHEM,W-1000 BERLIN,GERMANY

来源：

INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES | 1992年 / 14卷 / 01期

关键词：

METHIONINE SPECIFIC TRANSFER RNA; PLANT TRANSFER RNAIMET; PLANT RIBOSOMAL 5S RNA; HYDROXYL RADICALS; TRANSFER RNA TERTIARY STRUCTURE; 5S RIBOSOMAL-RNA TERTIARY STRUCTURE;

D O I：

10.1016/S0141-8130(05)80018-2

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Ribosomal 5S RNA is present in all eubacterial and eukaryotic ribosomes. Despite a large amount of experimental data on the primary and secondary structures of these types of molecules, details of their tertiary structure and their precise function in protein biosynthesis are still not known. Recently we have proposed a new model for the tertiary structure of plant 5S rRNA. In this study we applied the Fe(II)-mediated cleavage reaction to test the model. The data presented here provide experimental evidence that in the 5S rRNA molecule only a few nucleotides are buried in the tertiary structure. Similar experiments performed with methionine initiator tRNA gave results which imply the difference in its structure when compared with the X-ray structure of yeast tRNA(Phe).

引用

页码：41 / 44

页数：4

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