IDENTIFICATION AND CHARACTERIZATION OF STREPTOCOCCUS-THERMOPHILUS STRAINS BY PULSED-FIELD GEL-ELECTROPHORESIS

被引：17

作者：

BOUTROU, R ^{[1
]}

THUAULT, D ^{[1
]}

BOURGEOIS, CM ^{[1
]}

机构：

[1] ADRIA,F-29334 QUIMPER,FRANCE

来源：

JOURNAL OF APPLIED BACTERIOLOGY | 1995年 / 79卷 / 04期

关键词：

D O I：

10.1111/j.1365-2672.1995.tb03161.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The chromosomal DNA of a number of strains of the lactic acid bacterium Streptococcus thermophilus was analysed with the aim of rapidly differentiating and assessing the characteristics of each strain. Pulsed-field gel electrophoresis was used to separate large DNA fragments formed by the restriction enzymes SmaI, SfiI or ApaI. Hybridization with a non-radioactive DNA probe confirmed the identification of strains as Strep. thermophilus and analysis of the electrophoretic patterns differentiated some strains from others. A more extensive study of the pulsed-field electrophoresis restriction patterns of new isolates of Strep, thermophilus may facilitate assessment of their technological properties by comparison of their restriction patterns with those of reference strains.

引用

页码：454 / 458

页数：5

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