FIBROBLAST GROWTH-FACTOR IN THE HYPOTHALAMIC-PITUITARY AXIS - DIFFERENTIAL EXPRESSION OF FIBROBLAST GROWTH FACTOR-II AND A HIGH-AFFINITY RECEPTOR

被引:56
作者
GONZALEZ, AM
LOGAN, A
YING, WB
LAPPI, DA
BERRY, M
BAIRD, A
机构
[1] UNIV BIRMINGHAM, DEPT CLIN CHEM, BIRMINGHAM B15 2TT, ENGLAND
[2] UNITED MED & DENT SCH GUYS & ST THOMAS HOSP, DEPT ANAT & CELL BIOL, LONDON SE1 9RT, ENGLAND
关键词
D O I
10.1210/en.134.5.2289
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
In situ hybridization and immunohistochemistry were used to map gene expression and protein distribution of basic fibroblast growth factor (FGF-2) in the hypothalamic-pituitary system. Although the expression of FGF-2 mRNA in the pituitary is low, the protein is widely distributed in both its neural and anterior lobes. In the anterior lobe, immunoreactive (ir-) FGF-2 localizes to basement membranes and select endocrine cells. In the neural lobe, ir-FGF-2 is detected in basement membranes, pituicytes, and Herring bodies. Analyses of FGF high affinity receptor (FGFR) immunoreactivity in the anterior pituitary establishes a distribution of FGFR similar to that of FGF-2. In the neural lobe, ir-FGFR is associated with nerve fibers, pituicytes, and Herring bodies. Unlike FGF-2, the distribution of FGFR1 mRNA correlates well with the presence of the immunoreactive receptor. In the hypothalamus, magnocellular neurons of paraventricular and supraoptic nuclei contain ir-FGF-2 and ir-FGFR. In the median eminence, ir-FGF-2 and ir-FGFR is associated with fibers, glial, and endothelial cells. Ependymal and subependymal cells lining the third ventricle also show high levels of ir-FGF-2 and ir-FGFR and mRNAs. Overall, there is a specific and selective distribution of FGF-2 and its high affinity receptor(s) in the hypothalamo-pituitary axis. This localization lead us to postulate a role in neurohypophyseal functions, possibly water balance.
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页码:2289 / 2297
页数:9
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