PLATELET FACTOR STIMULATING HUMAN NORMAL GLIAL CELLS

被引：218

作者：

WESTERMARK, B

WASTESON, A

机构：

[1] UNIV UPPSALA, INST MED & PHYSIOL CHEM, S-75122 UPPSALA, SWEDEN

[2] UNIV UPPSALA, WALLENBERG LAB, S-75122 UPPSALA, SWEDEN

来源：

EXPERIMENTAL CELL RESEARCH | 1976年 / 98卷 / 01期

关键词：

D O I：

10.1016/0014-4827(76)90476-6

中图分类号：

R73 [肿瘤学];

学科分类号：

100214 ;

摘要：

Multiplication stimulating activities of human serum and fractions thereof were determined as stimulation of [3H]thymidine incorporation of serum-deprived human glial cells. Serum prepared from cell-free plasma had a considerably lower activity than serum prepared from whole blood. The major part of the growth-promoting activity of serum could be ascribed to a platelet factor, released during the coagulation process. The factor was trypsin-labile and heat-stable. A partial purification of the factor was achieved by ion exchange chromatography on carboxy methyl-Sephadex at neutral pH. The purified material was 600-700 times as active as normal human serum on a protein basis.

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收藏

页码：170 / 174

页数：5

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