OVEREXPRESSION OF L7/L12 PROTEIN WITH MUTATIONS IN ITS FLEXIBLE REGION

被引：25

作者：

GUDKOV, AT

BUBUNENKO, MG

GRYAZNOVA, OI

机构：

[1] Institute of Protein Research, Academy of Sciences, the USSR, Pushchino

来源：

BIOCHIMIE | 1991年 / 73卷 / 11期

关键词：

RIBOSOME; L7/L12; PROTEINS; MUTAGENESIS; PCR;

D O I：

10.1016/0300-9084(91)90168-Z

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Using restriction enzymes and polymerase chain reaction, three mutated forms of L7/L12 proteins with deleted 38-46. 44-52 and 38-52 residues were obtained. These mutant proteins were isolated in a preparative scale and were shown to bind to ribosomes and to affect ribosomal function.

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页码：1387 / 1389

页数：3

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