D-genome disomic substitution lines of durum wheat (Triticum turgidium L. var. durum) and their parental cultivars Langdon (recipient) and Chinese Spring (D-genome donor) were evaluated for their relative heat tolerance as measured by membrane thermostability to determine the chromosomal locations of genes controlling this trait. Results indicate that homoelogues 3 and 4 are most closely associated with heat tolerance. Chromosomes 3A, 3B, 4A, 4B, and 6A were found to be associated with heat tolerance of Langdon, while chromosomes 1B, 2A, 6B, and 7B were not related to heat tolerance.