ISOLATION OF CDNA OF AN AUXIN-REGULATED GENE ENCODING A G-PROTEIN BETA-SUBUNIT-LIKE PROTEIN FROM TOBACCO BY-2-CELLS

被引:108
作者
ISHIDA, S [1 ]
TAKAHASHI, Y [1 ]
NAGATA, T [1 ]
机构
[1] UNIV TOKYO,GRAD SCH SCI,DEPT PLANT SCI,BUNKYO KU,TOKYO 113,JAPAN
关键词
AUXIN STARVATION; TOBACCO SUSPENSION CULTURED CELL (BY-2); (TRP-ASP)-40 REPEAT; CELL DIVISION;
D O I
10.1073/pnas.90.23.11152
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
The addition of 2,4-dichlorophenoxyacetic acid to tobacco BY-2 cells that had been cultured in modified Linsmaier and Skoog medium deprived of auxin for 3 days induced cell division, whereas without 2,4-dichlorophenoxyacetic acid application, no such induction of cell division was seen. When differential cDNA screening for auxin was done at 4 hr after the addition of 2,4-dichlorophenoxyacetic acid, the cDNA of an auxin-responsive gene designated arcA was isolated. The predicted gene product of arc-4 is a polypeptide with a M(r) of 35,825. arcA, thus, is a plant hormone-regulated gene that encodes a protein structurally related to the beta subunit of a guanine nucleotide-binding regulatory protein, which is composed of seven repetitive segments of Trp-Asp 40-aa repeats. The possibility that arcA gene products induce cell division is discussed.
引用
收藏
页码:11152 / 11156
页数:5
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