INTEGRIN-BINDING PEPTIDE IN SOLUTION INHIBITS OR ENHANCES ENDOTHELIAL-CELL MIGRATION, PREDICTABLY FROM CELL-ADHESION

被引:50
作者
WU, P
HOYING, JB
WILLIAMS, SK
KOZIKOWSKI, BA
LAUFFENBURGER, DA
机构
[1] UNIV ILLINOIS,DEPT CHEM ENGN,297 ROGER ADAMS LAB,600 S MATHEWS ST,URBANA,IL 61801
[2] UNIV ILLINOIS,DEPT CELL & STRUCT BIOL,URBANA,IL 61801
[3] UNIV ARIZONA,DEPT SURG,TUCSON,AZ 85721
[4] PROCTER & GAMBLE CO,DEPT RES & DEV,CINCINNATI,OH 45239
关键词
ADHESION RECEPTORS; ECHISTATIN;
D O I
10.1007/BF02390372
中图分类号
R318 [生物医学工程];
学科分类号
0831 ;
摘要
We have examined the effects of an integrin-binding competitor, echistatin, in solution on adhesion and migration of rat microvessel endothelial cells on fibronectin in vitro. A biphasic dependence of cell motility on fibronectin surface density was observed, with a peak random motility coefficient of about 8 x 10(-9) cm2/sec occurring below 0.3 mug/cm2 fibronectin. In the presence of echistatin at 0.5 mum, the peak random motility coefficient was similar but occurred at the significantly greater fibronectin surface density of 1.2 mug/cm2. Hence, the same concentration of this soluble integrin-binding competitor inhibited migration on low fibronectin densities but enhanced migration on high fibronectin densities. At the same time, echistatin decreased adhesiveness on all fibronectin surface densities. When motility was correlated explicitly with adhesiveness, a single biphasic relationship was obtained for both absence and presence of echistatin with peak motility occuring in both cases at identical adhesiveness. Both the inhibiting and enhancing effects of the soluble integrin-binding competitor on motility are predictable from its effect on adhesion, consistent with the theoretical models of Lauffenburger (15) and DiMilla et al. (3).
引用
收藏
页码:144 / 152
页数:9
相关论文
共 29 条
[1]   BIOLOGICALLY-ACTIVE SYNTHETIC PEPTIDES AS PROBES OF EMBRYONIC-DEVELOPMENT - A COMPETITIVE PEPTIDE INHIBITOR OF FIBRONECTIN FUNCTION INHIBITS GASTRULATION IN AMPHIBIAN EMBRYOS AND NEURAL CREST CELL-MIGRATION IN AVIAN EMBRYOS [J].
BOUCAUT, JC ;
DARRIBERE, T ;
POOLE, TJ ;
AOYAMA, H ;
YAMADA, KM ;
THIERY, JP .
JOURNAL OF CELL BIOLOGY, 1984, 99 (05) :1822-1830
[2]  
CHU L, UNPUB AICHE J
[3]   ADSORPTION AND ELUTION OF EXTRACELLULAR-MATRIX PROTEINS ON NONTISSUE CULTURE POLYSTYRENE PETRI DISHES [J].
DIMILLA, PA ;
ALBELDA, SM ;
QUINN, JA .
JOURNAL OF COLLOID AND INTERFACE SCIENCE, 1992, 153 (01) :212-225
[4]   MAXIMAL MIGRATION OF HUMAN SMOOTH-MUSCLE CELLS ON FIBRONECTIN AND TYPE-IV COLLAGEN OCCURS AT AN INTERMEDIATE ATTACHMENT STRENGTH [J].
DIMILLA, PA ;
STONE, JA ;
QUINN, JA ;
ALBELDA, SM ;
LAUFFENBURGER, DA .
JOURNAL OF CELL BIOLOGY, 1993, 122 (03) :729-737
[5]   MATHEMATICAL-MODEL FOR THE EFFECTS OF ADHESION AND MECHANICS ON CELL-MIGRATION SPEED [J].
DIMILLA, PA ;
BARBEE, K ;
LAUFFENBURGER, DA .
BIOPHYSICAL JOURNAL, 1991, 60 (01) :15-37
[6]   ARGINYL-GLYCYL-ASPARTIC ACID (RGD) - A CELL-ADHESION MOTIF [J].
DSOUZA, SE ;
GINSBERG, MH ;
PLOW, EF .
TRENDS IN BIOCHEMICAL SCIENCES, 1991, 16 (07) :246-250
[7]   CELL-ADHESION MOLECULES - IMPLICATIONS FOR A MOLECULAR HISTOLOGY [J].
EDELMAN, GM ;
CROSSIN, KL .
ANNUAL REVIEW OF BIOCHEMISTRY, 1991, 60 :155-190
[8]   QUANTITATIVE RELATIONSHIPS BETWEEN SINGLE-CELL AND CELL-POPULATION MODEL PARAMETERS FOR CHEMOSENSORY MIGRATION RESPONSES OF ALVEOLAR MACROPHAGES TO C5A [J].
FARRELL, BE ;
DANIELE, RP ;
LAUFFENBURGER, DA .
CELL MOTILITY AND THE CYTOSKELETON, 1990, 16 (04) :279-293
[9]   REGULATION OF INVITRO CAPILLARY-TUBE FORMATION BY ANTI-INTEGRIN ANTIBODIES [J].
GAMBLE, JR ;
MATTHIAS, LJ ;
MEYER, G ;
KAUR, P ;
RUSS, G ;
FAULL, R ;
BERNDT, MC ;
VADAS, MA .
JOURNAL OF CELL BIOLOGY, 1993, 121 (04) :931-943
[10]   THE E8 SUBFRAGMENT OF LAMININ PROMOTES LOCOMOTION OF MYOBLASTS OVER EXTRACELLULAR-MATRIX [J].
GOODMAN, SL ;
RISSE, G ;
VONDERMARK, K .
JOURNAL OF CELL BIOLOGY, 1989, 109 (02) :799-809