COMPARATIVE IMMUNOREACTIVITY OF THE EOSINOPHIL CONSTITUENTS MBP AND ECP IN DIFFERENT TYPES OF URTICARIA

被引:43
作者
HAAS, N
MOTEL, K
CZARNETZKI, BM
机构
[1] FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,HAUT POLIKLIN,D-13353 BERLIN,GERMANY
[2] FREE UNIV BERLIN,KLINIKUM RUDOLF VIRCHOW,ASTHMA POLIKLIN,D-13353 BERLIN,GERMANY
关键词
URTICARIA; EOSINOPHILS; MBP; ECP; FC-EPSILON-RII;
D O I
10.1007/BF01262329
中图分类号
R75 [皮肤病学与性病学];
学科分类号
100206 ;
摘要
In order to elucidate the role of eosinophil constituents in urticaria, we investigated major basic protein expression immunohistologically in comparison with that of eosinophilic cationic protein and the low-affinity IgE receptor in lesional and uninvolved skin of different types of urticaria. Eosinophil activation was studied with the markers EG1 and EG2. Different eosinophil constituents were found in all urticarial lesions except those of urticaria pigmentosa. MBP staining tended to be distributed diffusely throughout the tissue, whereas EG1 and EG2 antibodies were located at or close to individual cells. Staining with the low affinity IgE receptor antibody was rare. In uninvolved skin, major basic protein and particularly eosinophilic cationic protein reactivity was found in chronic recurrent urticaria, delayed pressure urticaria and, to a minor degree, in cholinergic urticaria. No correlation was found between antibody reactivity and eosinophil counts. Reactivity with either of the eosinophil constituents is thus a better marker for eosinophil involvement than routine H&E staining of the cells. The demonstration of eosinophil constituents in nonlesional skin of some urticaria patients suggests generalized eosinophil activation in certain subtypes of the disease.
引用
收藏
页码:180 / 185
页数:6
相关论文
共 29 条
[1]  
BUTTERFIELD JH, 1990, LAB INVEST, V62, P77
[2]   CURRENT CONCEPTS - EOSINOPHIL FUNCTION [J].
BUTTERWORTH, AE ;
DAVID, JR .
NEW ENGLAND JOURNAL OF MEDICINE, 1981, 304 (03) :154-156
[3]   MORPHOLOGY OF THE CELLULAR INFILTRATE IN DELAYED PRESSURE URTICARIA [J].
CZARNETZKI, BM ;
MEENTKEN, J ;
KOLDE, G ;
BROCKER, EB .
JOURNAL OF THE AMERICAN ACADEMY OF DERMATOLOGY, 1985, 12 (02) :253-259
[4]   IMMUNOHISTOCHEMICAL DEMONSTRATION OF MIGRATION-INHIBITORY FACTOR IN DIFFERENT TYPES OF URTICARIA [J].
CZARNETZKI, BM ;
ZWADLOKLARWASSER, G ;
BROCKER, EB ;
SORG, C .
JOURNAL OF INVESTIGATIVE DERMATOLOGY, 1989, 93 (04) :471-474
[5]   COMPARATIVE STUDIES OF HUMAN EOSINOPHIL MIGRATION TOWARDS PLATELET-ACTIVATING FACTOR AND LEUKOTRIENE-B4 [J].
CZARNETZKI, BM ;
CSATO, M .
INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY, 1989, 88 (1-2) :191-193
[6]  
CZARNETZKI BM, 1989, DERMATOLOGICA, V179, pS54
[7]  
FUJISAWA T, 1990, J IMMUNOL, V144, P642
[8]  
GLEICH GJ, 1986, ANN INST PASTEUR IMM, VD137, P136
[9]   DEMONSTRATION OF THE HIGH-AFFINITY IGE RECEPTOR ON HUMAN LANGERHANS CELLS IN NORMAL AND DISEASED SKIN [J].
GRABBE, J ;
HAAS, N ;
HAMANN, K ;
KOLDE, G ;
HAKIMI, J ;
CZARNETZKI, BM .
BRITISH JOURNAL OF DERMATOLOGY, 1993, 129 (02) :120-123
[10]  
GREAVES MW, 1991, J R COLL PHYSICIANS, V26, P199