KINETIC-STUDIES ON THE FORMATION OF ACRIDINE-LINKED DNA TRIPLE HELICES

被引：21

作者：

FOX, KR

机构：

[1] Department of Physiology and Pharmacology, University of Southampton, Southampton, SO16 7PX, Bassett Crescent East

来源：

FEBS LETTERS | 1995年 / 357卷 / 03期

关键词：

TRIPLE HELIX; ASSOCIATION KINETICS; DNASE I FOOTPRINTING;

D O I：

10.1016/0014-5793(94)01387-G

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have used DNase I footprinting to measure the rate of intermolecular triple helix formation at the target sites A(6)G(6) . C6T6 and G(6)A(6) . T6C6 with the acridine-linked oligonucleotides Acr-T5C5 and Acr-C5T5, respectively. Under pseudo first-order reaction conditions we find that the reactions are slow, with half-lives of several minutes. The rates are dependent on the concentration of the third strand oligonucleotide and yield bimolecuIar association rate constants of 300 M(-1) s(-1) for Acr-T5C5 binding to A(6)G(6) . C6T6 and 2000 M(-1) s(-1) for the interaction of Acr-C5T5 with G(6)A(6) . T6C6.

引用

页码：312 / 316

页数：5

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