INVOLVEMENT OF CD5 IN T(H)1 AND T(H)2 CONTACT-MEDIATED RESCUE OF ANTI-MU AND IONOMYCIN INDUCED GROWTH-INHIBITION IN A B-CELL LYMPHOMA

Cross-linking of membrane Ig receptors by anti-mu antibodies (Ab) or treatment with ionomycin induced complete growth arrest and subsequent apoptotic cell death in an immature B cell lymphoma, BKS-2. The growth-inhibitory signals delivered by anti-mu and ionomycin were overcome by anti-CD3-activated T(h)2 clones D10.G4 and F1 and by T(h)1 cell clone S53. In this report the T-h-mediated growth reversal in BKS-2 cells was shown to require contact-dependent interactions when the inhibition was caused by immobilized anti-mu or ionomycin. T(h)2 cells in transwells (lymphokines) failed to protect BKS-2 cells from the growth-inhibitory effect of immobilized anti-mu or ionomycin. Monoclonal antibodies to CD5 or CD40 ligands on activated T-h cells partially inhibited the T(h)2 contact-dependent growth reversal of BKS-2 cells whereas simultaneous addition of both antibodies effectively prevented the delivery of contact-mediated growth signal. In contrast, anti-class I or class II Ab did not affect T-h cell mediated growth reversal of BKS-2 cells. These data demonstrated that noncognate physical interaction with T-h cells was essential for the recovery of BKS-2 cells when the latter were growth-arrested by strong inhibitory stimuli such as immobilized anti-mu and ionomycin. Further CD5 as well as CD40 ligands on T-h cells are important for signal transduction in this type of T-B interaction.