ROLE OF AN N-ETHYLMALEIMIDE-SENSITIVE TRANSPORT COMPONENT IN PROMOTING FUSION OF TRANSPORT VESICLES WITH CISTERNAE OF THE GOLGI STACK

被引：332

作者：

MALHOTRA, V

ORCI, L

GLICK, BS

BLOCK, MR

ROTHMAN, JE

机构：

[1] STANFORD UNIV, DEPT BIOCHEM, STANFORD, CA 94305 USA

[2] UNIV GENEVA, SCH MED, INST HISTOL & EMBRYOL, DEPT MORPHOL, CH-1211 GENEVA 4, SWITZERLAND

来源：

CELL | 1988年 / 54卷 / 02期

关键词：

D O I：

10.1016/0092-8674(88)90554-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

An N-ethylmaleimide-sensitive transport component (NSF) has been purified on the basis of its ability to support transport between Golgi cisternae. We now report that NSF is needed for membrane fusion. Thus, when NSF is withheld from incubations of Golgi stacks with cytosol and ATP, uncoated transport vesicles accumulate. Biochemical experiments confirm this conclusion and reveal that NSF is needed to form the first of two previously described perfusion complexes. NSF, therefore, acts within a cascade in which a vesicle-cisterna complex is matured until it is competent for fusion. We suggest that this reflects the stepwise assembly of a multisubunit "fusion machine" following vesicle attachment.

引用

页码：221 / 227

页数：7

共 17 条

[1]

ALBERTS BM, 1984, COLD SPRING HARB SYM, V49, P1

[2] RECONSTITUTION OF THE TRANSPORT OF PROTEIN BETWEEN SUCCESSIVE COMPARTMENTS OF THE GOLGI MEASURED BY THE COUPLED INCORPORATION OF N-ACETYLGLUCOSAMINE [J].