KINETIC-STUDY OF THE PERFORMANCE OF 3RD-GENERATION BIOSENSORS

被引：29

作者：

KOOPAL, CGJ ^{[1
]}

NOLTE, RJM ^{[1
]}

机构：

[1] UNIV NIJMEGEN,NIJMEGEN SON RES CTR,6525 ED NIJMEGEN,NETHERLANDS

来源：

BIOELECTROCHEMISTRY AND BIOENERGETICS | 1994年 / 33卷 / 01期

关键词：

D O I：

10.1016/0302-4598(94)87031-4

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A kinetic study of the performance of third-generation biosensors for glucose based on glucose oxidase immobilized on a microporous matrix of the conducting polymer poly(pyrrole) is presented. The mechanism of the enzymatically catalyzed oxidation of glucose will be different in this type of biosensor as the natural electron acceptor oxygen is replaced by the conducting polymer. Different kinetic parameters are found for the immobilized glucose oxidase than for the enzyme in solution. Mediation by the conducting polymer is found to be very effective and no significant electron transfer to oxygen is observed. In addition to substrate transport limitation in the microporous matrix, the enzymatic reaction in the biosensors is limited by the applied potential.

引用

页码：45 / 53

页数：9

共 53 条

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