CONDITIONS FAVORABLE FOR THE SOMATIC EMBRYOGENESIS IN CARROT CELL-CULTURE ENHANCE EXPRESSION OF THE ROLC PROMOTER-GUS FUSION GENE

被引：19

作者：

FUJII, N

UCHIMIYA, H

机构：

[1] HOKKAIDO UNIV,FAC SCI,DEPT BOT,SAPPORO 604,JAPAN

[2] UNIV TSUKUBA,INST BIOL SCI,TSUKUBA,IBARAKI 305,JAPAN

来源：

PLANT PHYSIOLOGY | 1991年 / 95卷 / 01期

关键词：

D O I：

10.1104/pp.95.1.238

中图分类号：

Q94 [植物学];

学科分类号：

071001 ;

摘要：

We obtained carrot (Daucus carota) cells possessing the 5'-noncoding sequence of the ORF12 gene (rolC) of TL-DNA of the Ri plasmid and a structural gene of bacterial beta-glucuronidase by Agrobacterium-mediated transformation. When such cells were cultured in medium containing 2,4-dichlorophenoxyacetic acid, substantial reduction in beta-glucuronidase activity was observed. Upon transferring the cells from a 2,4-D-containing medium to one devoid of 2,4-dichlorophenoxyacetic acid, enhanced expression of beta-glucuronidase in somatic embryo development was recorded. Activation by gibberillic acid and suppression by abscisic acid of beta-glucuronidase activities, in concord with embryogenesis, were also noted.

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页码：238 / 241

页数：4

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