Determination of intramolecular hydrogen bonds in amikacin in water solution by NMR spectroscopy

被引：14

作者：

Gaggelli, E

Gaggelli, N

Maccotta, A

Valensin, G

Marini, D

DiCocco, ME

Delfini, M

机构：

[1] LAB CHIM DOGANE,ROME,ITALY

[2] UNIV ROMA LA SAPIENZA,DEPT CHEM,I-00195 ROME,ITALY

来源：

SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY | 1995年 / 51卷 / 11期

关键词：

D O I：

10.1016/0584-8539(95)01548-9

中图分类号：

O433 [光谱学];

学科分类号：

0703 ; 070302 ;

摘要：

An NMR investigation has been carried out on amikacin in water solution in the physiological pH range. Two-dimenstional heterocorrelated maps provide H-1 NMR chemical shifts from the unambiguous assignment of the C-13 NMR spectrum. Reorientational dynamics at the molecular level are interpreted in terms of a pseudoisotropic motion with a correlation time of 0.17 ns at 300 K, The pH and temperature dependences of C-13 NMR chemical shifts are interpreted to delineate protonation equilibria (all pKs are determined) and to assess the occurrence of two intermolecular hydrogen bonds, which are confirmed by molecular modelling.

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页码：1959 / 1963

页数：5

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