AN IMPROVED METHOD FOR ISOLATING CARDIAC MYOCYTES USEFUL FOR PATCH-CLAMP STUDIES

被引：94

作者：

YAZAWA, K

KAIBARA, M

OHARA, M

KAMEYAMA, M

机构：

[1] National Institute for Physiological Sciences, Okazaki

[2] Department of Pharmacology, Asahikawa Medical College, Asahikawa

[3] Department of Internal Medicine, Nagasaki University, School of Medicine, Nagasaki

来源：

JAPANESE JOURNAL OF PHYSIOLOGY | 1990年 / 40卷 / 01期

关键词：

dissociation of cells; isolated myocytes; patch clamp;

D O I：

10.2170/jjphysiol.40.157

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

An improved method for isolating cardiac myocytes is described. This method consists of the initial dispersion of the myocytes with a new type of collagenase and the following treatment of the cells with a non-specific protease. This method gives more than 80% rod-shaped, viable, and quiescent cells, which are suitable for biochemical as well as electrophysiological experiments. Formation of a GΩ seal and subsequent whole-cell recording is easily performed using these cells. © 1990, PHYSIOLOGICAL SOCIETY OF JAPAN. All rights reserved.

引用

页码：157 / 163

页数：7

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