ESTIMATING GENOMIC DISTANCE FROM DNA-SEQUENCE LOCATION IN CELL-NUCLEI BY A RANDOM-WALK MODEL

被引：219

作者：

VANDENENGH, G

SACHS, R

TRASK, BJ

机构：

[1] UNIV WASHINGTON,SCH MED,DEPT MOLEC BIOTECHNOL GJ-10,SEATTLE,WA 98195

[2] UNIV CALIF BERKELEY,DEPT MATH,BERKELEY,CA 94720

[3] LAWRENCE LIVERMORE NATL LAB,DIV BIOMED SCI,CTR HUMAN GENOME,LIVERMORE,CA 94550

来源：

SCIENCE | 1992年 / 257卷 / 5075期

关键词：

D O I：

10.1126/science.1388286

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

The folding of chromatin in interphase cell nuclei was studied by fluorescent in situ hybridization with pairs of unique DNA sequence probes. The sites of DNA sequences separated by 100 to 2000 kilobase pairs (kbp) are distributed in interphase chromatin according to a random walk model. This model provides the basis for calculating the spacing of sequences along the linear DNA molecule from interphase distance measurements. An interphase mapping strategy based on this model was tested with 13 probes from a 4-megabase pair (Mbp) region of chromosome 4 containing the Huntington disease locus. The results confirmed the locations of the probes and showed that the remaining gap in the published maps of this region is negligible in size. Interphase distance measurements should facilitate construction of chromosome maps with an average marker density of one per 100 kbp, approximately ten times greater than that achieved by hybridization to metaphase chromosomes.

引用

页码：1410 / 1412

页数：3

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