THE DNA-POLYMERASE GENE FROM CHLORELLA VIRUSES PBCV-1 AND NY-2A CONTAINS AN INTRON WITH NUCLEAR SPLICING SEQUENCES

被引:51
作者
GRABHERR, R [1 ]
STRASSER, P [1 ]
VANETTEN, JL [1 ]
机构
[1] UNIV NEBRASKA, DEPT PLANT PATHOL, LINCOLN, NE 68583 USA
关键词
D O I
10.1016/0042-6822(92)90527-V
中图分类号
Q93 [微生物学];
学科分类号
071005 ; 100705 ;
摘要
The deduced amino acid sequences of two eukaryotic chlorella virus (PBCV-1 and NY-2A) DNA polymerases are 90% identical and contain amino acid motifs typical of alpha-like (Family B) DNA polymerases. The open reading frames of both PBCV-1 and NY-2A DNA polymerases are interrupted by an identically located, small (101 or 86 nucleotides, respectively) intron that resembles eukaryotic nuclear-spliced messenger RNA introns. This discovery suggests that chlorella virus replication has a nuclear phase. © 1992.
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页码:721 / 731
页数:11
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