SECRETION OF SINGLE-CHAIN UROKINASE-TYPE PLASMINOGEN-ACTIVATOR FROM INSECT CELLS

被引：7

作者：

KING, LA ^{[1
]}

KAUR, K ^{[1
]}

MANN, SG ^{[1
]}

LAWRIE, AM ^{[1
]}

STEVEN, J ^{[1
]}

OGDEN, JE ^{[1
]}

机构：

[1] DELTA BIOTECHNOL LTD,NOTTINGHAM NG7 1FD,ENGLAND

来源：

GENE | 1991年 / 106卷 / 02期

关键词：

BACULOVIRUS; AUTOGRAPHA-CALIFORNICA NUCLEAR POLYHEDROSIS VIRUS; POLYHEDRON PROMOTER; SPODOPTERA-FRUGIPERDA CELLS; RECOMBINANT DNA;

D O I：

10.1016/0378-1119(91)90194-G

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A cDNA encoding human urokinase-type plasminogen activator was inserted downstream from the polyhedrin promoter of the baculovirus Autographa californica nuclear polyhedrosis virus. A protein of similar M(r) to urokinase (UK) was synthesized and approx. 90% was secreted from recombinant virus-infected Spodoptera frugiperda cells. Zymography and Western blotting analysis of the insect-derived protein demonstrated that it was comprised solely of the high-M(r) form of UK. No low-M(r) UK was detected. Amidolytic activity assays showed that 96% of the insect cell-derived UK was in the single-chain proenzyme form. The yield of UK from insect cells was 1986 international units/Ml/10(6) infected cells.

引用

页码：151 / 157

页数：7

共 30 条

[1] BACULOVIRUSES AS VECTORS FOR FOREIGN GENE-EXPRESSION IN INSECT CELLS [J].