PHARMACOLOGICAL CHARACTERIZATION OF ADENOSINE-A1 AND A2 RECEPTORS IN THE BLADDER - EVIDENCE FOR A MODULATORY ADENOSINE TONE REGULATING NONADRENERGIC NONCHOLINERGIC NEUROTRANSMISSION

被引:21
作者
ACEVEDO, CG
CONTRERAS, E
ESCALONA, J
LEWIN, J
HUIDOBROTORO, JP
机构
[1] PONTIFICIA UNIV CATOLICA CHILE, FAC BIOL SCI,DEPT PHYSIOL, NEUROHUMORAL REGULAT UNIT,POB 114-D, SANTIAGO, CHILE
[2] UNIV CONCEPCION, FAC BIOL SCI & NAT RESOURCES, DEPT PHARMACOL, CONCEPCION, CHILE
关键词
NONNORADRENERGIC NONCHOLINERGIC NEUROTRANSMISSION; PURINERGIC TRANSMISSION; ADENOSINE RECEPTORS; ADENOSINE A1; A2; MECHANISMS; ADENOSINE MODULATION; URINARY BLADDER;
D O I
10.1111/j.1476-5381.1992.tb14473.x
中图分类号
R9 [药学];
学科分类号
1007 ;
摘要
1 The nerve-evoked contractions elicited by transmural electrical stimulation of mouse urinary bladders superfused in modified Krebs Ringer buffer containing 1-mu-M atropine plus 3.4-mu-M guanethidine were inhibited by adenosine (ADO) and related nucleoside analogues with the following rank order of potency: RB-phenylisopropyladenosine (RB-PIA) > cyclohexyladenosine (CHA) > 5'N-ethylcarboxamido adenosine (NECA) > ADO > S-phenylisopropyladenosine (S-PIA). Tissue preincubation with 8-phenyltheophylline (8-PT) displaced to the right, in a parallel fashion, the NECA concentration-response curve. 2 The contractions elicited by application of exogenous adenosine 5'-triphosphate (ATP) were also inhibited by ADO and related structural analogues. The rank order of potency to reduce the motor response to ATP was: NECA > 2-chloroadenosine (CADO) > RB-PIA > ADO > CHA > S-PIA. 3 The ADO-induced ATP antagonism was of a non-competitive nature and was not specific. Tissue incubation with 10-mu-m NECA not only reduced the motor responses elicited by ATP, but also 5-hydroxytryptamine, acetylcholine and prostaglandin F2-alpha. The action of NECA was antagonized following tissue preincubation with 8-PT. The inhibitory action of NECA was not mimicked by 10-mu-m CHA. 4 The maximal bladder ATP contractile response was significantly increased by tissue preincubation with 5-30-mu-m 8-PT. 5 The 0.15 Hz evoked muscular twitch was significantly increased by 8-PT while dipyridamole consistently reduced the magnitude of the twitch response. These results are consonant with the hypothesis that an endogenous ADO tone modulates the bladder neurotransmission. 6 A working model is proposed suggesting the presence of ADO-A1 and A2 receptors in the mouse urinary bladder. The A1 receptor subpopulation is probably of presynaptic origin whereas the smooth muscle membranes contain a population of the A2 receptor subtype.
引用
收藏
页码:120 / 126
页数:7
相关论文
共 38 条
[1]   BRADYKININ FACILITATES THE PURINERGIC MOTOR COMPONENT OF THE RAT BLADDER NEUROTRANSMISSION [J].
ACEVEDO, CG ;
LEWIN, J ;
CONTRERAS, E ;
HUIDOBROTORO, JP .
NEUROSCIENCE LETTERS, 1990, 113 (02) :227-232
[2]   POSSIBLE INVOLVEMENT OF ADENINE-NUCLEOTIDES IN THE NEUROTRANSMISSION OF THE MOUSE URINARY-BLADDER [J].
ACEVEDO, CG ;
CONTRERAS, E .
COMPARATIVE BIOCHEMISTRY AND PHYSIOLOGY C-TOXICOLOGY & PHARMACOLOGY, 1985, 82 (02) :357-361
[3]   THE RAPID UPTAKE AND RELEASE OF [H-3]ADENOSINE BY RAT CEREBRAL CORTICAL SYNAPTOSOMES [J].
BENDER, AS ;
WU, PH ;
PHILLIS, JW .
JOURNAL OF NEUROCHEMISTRY, 1981, 36 (02) :651-660
[4]   EFFECTS OF ADENOSINE 5'-TRIPHOSPHATE (ATP) AND BETA-GAMMA-METHYLENE ATP ON THE RAT URINARY-BLADDER [J].
BROWN, C ;
BURNSTOCK, G ;
COCKS, T .
BRITISH JOURNAL OF PHARMACOLOGY, 1979, 65 (01) :97-102
[5]   ATROPINE RESISTANT EXCITATION OF URINARY-BLADDER - POSSIBILITY OF TRANSMISSION VIA NERVES RELEASING A PURINE NUCLEOTIDE [J].
BURNSTOCK, G ;
SMYTHE, A ;
DUMSDAY, B .
BRITISH JOURNAL OF PHARMACOLOGY, 1972, 44 (03) :451-+
[6]   PURINERGIC INNERVATION OF GUINEA-PIG URINARY-BLADDER [J].
BURNSTOCK, G ;
COCKS, T ;
CROWE, R ;
KASAKOV, L .
BRITISH JOURNAL OF PHARMACOLOGY, 1978, 63 (01) :125-138
[7]   DIRECT EVIDENCE FOR ATP RELEASE FROM NON-ADRENERGIC, NON-CHOLINERGIC (PURINERGIC) NERVES IN GUINEA-PIG TAENIA-COLI AND BLADDER [J].
BURNSTOCK, G ;
COCKS, T ;
KASAKOV, L ;
WONG, HK .
EUROPEAN JOURNAL OF PHARMACOLOGY, 1978, 49 (02) :145-149
[8]   IS THERE A BASIS FOR DISTINGUISHING 2 TYPES OF P2-PURINOCEPTOR [J].
BURNSTOCK, G ;
KENNEDY, C .
GENERAL PHARMACOLOGY, 1985, 16 (05) :433-440
[9]  
BURNSTOCK G, 1972, PHARMACOL REV, V24, P509
[10]  
BURNSTOCK G, 1990, ANN NY ACAD SCI, V603, P1