MULTIPLE FORMS OF BIOACTIVE AND IMMUNOREACTIVE ADRENOCORTICOTROPIN IN HUMAN PITUITARY AND BLOOD OF PATIENTS WITH NELSONS SYNDROME

被引:8
作者
GOVERDE, HJM
MARTENS, GJM
PESMAN, GJ
SMALS, AGH
机构
[1] CATHOLIC UNIV NIJMEGEN, FAC MED, DEPT ANIM PHYSIOL, 6525 GA NIJMEGEN, NETHERLANDS
[2] CATHOLIC UNIV NIJMEGEN, DEPT ENDOCRINOL & REPROD 3, 6525 GA NIJMEGEN, NETHERLANDS
[3] CATHOLIC UNIV NIJMEGEN, DIV CLIN ENDOCRINOL, 6525 GA NIJMEGEN, NETHERLANDS
关键词
D O I
10.1210/jc.77.2.443
中图分类号
R5 [内科学];
学科分类号
1002 ; 100201 ;
摘要
A pool of human pituitaries obtained from allegedly healthy subjects (traffic victims) and plasma samples from patients with Nelson's syndrome were analyzed by high performance liquid chromatography, and the corticosteroidogenic bioactivity and ACTH immunoreactivity were measured. Three bioactive forms of ACTH were detected in plasma samples and pituitary extract. The major form (peak III) coeluted with human ACTH-(1-39), showed a bioactive to immunoreactive ratio (B/I ratio) of about I, and represented about 80% of the total bioactivity in both the plasma samples and the pituitary extract. Peak I, with a B/I ratio greater than I, represented about 5%, and peak II, with a highly variable B/I ratio, represented about 7% of the bioactivity in both the plasma and pituitary extracts. A fraction with a very low B/I ratio was found to coelute with corticotropin-like intermediate lobe peptide. These data suggest that in Nelson's syndrome, ACTH secretion by the pituitary gland does not differ from that in normal subjects, at least qualitatively.
引用
收藏
页码:443 / 447
页数:5
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