INCOMPATIBILITY PROPERTIES OF THE NARROW-HOST-RANGE LACTOCOCCAL PLASMID PCI305

被引：5

作者：

FOLEY, S

DALY, C

FITZGERALD, GF

机构：

[1] NATL UNIV IRELAND UNIV COLL CORK,DEPT FOOD MICROBIOL,CORK,IRELAND

[2] NATL UNIV IRELAND UNIV COLL CORK,NATL FOOD BIOTECHNOL CTR,CORK,IRELAND

来源：

JOURNAL OF GENERAL MICROBIOLOGY | 1993年 / 139卷

关键词：

D O I：

10.1099/00221287-139-6-1271

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The cryptic plasmid pCI305 from Laetococcus lactis subsp. lactis UC317 was screened for incompatibility with a range of cloning vectors and was found to be incompatible with the theta-replicating plasmids pAM401 and pIL253. In contrast, lactococcal vectors containing the replication functions of the rolling circle replicating plasmids pSH71 and pWV01 were compatible with pCI305. A number of native lactococcal plasmids were also screened for both incompatibility and DNA sequence homology to the pCI305 replicon. A plasmid with high sequence identity was identified which was compatible with pCI305.

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页码：1271 / 1276

页数：6

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