ENZYMATICALLY ACTIVE ZINC, COPPER AND MERCURY DERIVATIVES OF THE ONE-IRON FORM OF PIG ALLANTOIC FLUID ACID-PHOSPHATASE

被引：79

作者：

BECK, JL ^{[1
]}

KEOUGH, DT ^{[1
]}

DEJERSEY, J ^{[1
]}

ZERNER, B ^{[1
]}

机构：

[1] UNIV QUEENSLAND, DEPT BIOCHEM, ST LUCIA, QLD 4067, AUSTRALIA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1984年 / 791卷 / 03期

关键词：

D O I：

10.1016/0167-4838(84)90347-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Derivatives of the violet, Fe-containing acid phosphatase of pig allantoic fluid were prepared in which 1 of the 2 iron atoms present in the native enzyme was replaced by Zn, Cu or Hg. The derivatives so formed are enzymatically active: the Zn-Fe, Cu-Fe and Hg-Fe enzymes have specific activities of about 80%, 25% and 17% respectively, of the maximum specific activity of the Fe-Fe enzyme in the standard assay at pH 4.9 with p-nitrophenyl phosphate as substrate. In contrast to the Fe-Fe enzyme, the mixed metal derivatives are not rapidly inactivated by H2O2. Visible absorption spectra of the derivatives confirm that all of the visible absorption of the Fe-Fe enzyme is due to 1 of the Fe atoms. Attempts to prepare an active Cu-Cu enzyme were unsuccessful.

引用

页码：357 / 363

页数：7

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