MOLECULAR-CLONING AND CHARACTERIZATION OF HUMAN PYRUVATE DEHYDROGENASE-BETA SUBUNIT GENE

被引：17

作者：

KOIKE, K

URATA, Y

KOIKE, M

机构：

[1] Dept. of Pathological Biochemistry, Atomic Disease Institute, Nagasaki Univ. School of Medicine

来源：

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA | 1990年 / 87卷 / 15期

关键词：

DNA sequence; Intron-exon junctions; Promoter; Thiamin pyrophosphate-dependent enzyme;

D O I：

10.1073/pnas.87.15.5594

中图分类号：

O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];

学科分类号：

07 ; 0710 ; 09 ;

摘要：

A genomic clone encompassing the entire gene for the human pyruvate dehydrogenase β subunit (PDHβ) has been isolated by screening a leukocyte genomic library with a nick-translated human foreskin fibroblast PDHβ cDNA probe. The 18-kilobase clone was characterized by restriction enzyme analysis, extensive DNA sequencing, and primer-extension analysis. The PDHβ structural gene is composed of 10 exons and 9 introns. All intron-exon splice junctions follow the GT/AG rule. The Alu family was found in introns 2 and 8. The 5' flanking region of the PDHβ gene contains a "CAAT" consensus promoter sequence but no "TATA" sequence. Primer-extension analysis indicated that the PDHβ gene transcription start site is an adenine residue located 132 bases upstream from the initiation codon in exon 1.

引用

页码：5594 / 5597

页数：4

共 19 条

[1] PURIFICATION OF BIOLOGICALLY-ACTIVE GLOBIN MESSENGER-RNA BY CHROMATOGRAPHY ON OLIGOTHYMIDYLIC-ACID-CELLULOSE [J].