PREMATURE CHROMOSOME CONDENSATION AS A SIGN OF OOCYTE IMMATURITY

被引：41

作者：

CALAFELL, JM

BADENAS, J

EGOZCUE, J

SANTALO, J

机构：

[1] Dept. Biologia Cellular i Fisiologia, Universitat Autònoma de Barcelona, 08193 Bellaterra, Barcelona

来源：

HUMAN REPRODUCTION | 1991年 / 6卷 / 07期

关键词：

IVF; OOCYTE IMMATURITY; PREMATURE CHROMOSOME CONDENSATION;

D O I：

10.1093/oxfordjournals.humrep.a137452

中图分类号：

R71 [妇产科学];

学科分类号：

100211 ;

摘要：

In this work we report the possibility that oocyte immaturity is associated with premature chromosome condensation (PCC) after in-vitro fertilization (IVF). Using a murine model, we have related PCC and endoreduplicated-like oocytes to oocyte immaturity as a basis for a prognosis in oocyte immaturity problems. The cytogenetic analysis was performed in 511 embryos obtained from immature oocytes that were directly fertilized in vitro and in 1363 embryos obtained from immature oocytes that were matured in vitro with different concentrations of human chorionic gonadotrophin (HCG) added to the culture medium. As a control we used 507 embryos obtained from freshly ovulated oocytes. PCC at the G1-phase-(G1-PCC) was observed only when immature oocytes were immediately fertilized in vitro (45.4%) and PCC at the S-phase (S-PCC) only when using in-vitro matured oocytes with the highest HCG concentration (3.3%). Neither G1-PCC nor S-PCC were found in the control group. Endoreduplicated-like oocytes appeared in a significant percentage (27.3%) only in the immature group. Immature oocytes yielded a low fertilization rate (16.6%) while in-vitro maturation seemed to confer a higher fertilization capacity compared to the control group (90.1% versus 78.2%). The possible correlation between PCC and oocyte immaturity provides new prospects in the determination of human IVF failures of unknown origin. Thus, when a problem of oocyte immaturity is diagnosed through the presence of PCC, a special programme of in vitro oocyte maturation, such as a longer preincubation time or addition of hormones to the media, would be recommended.

引用

页码：1017 / 1021

页数：5

共 21 条

[1]

Badenas J., Santalo J., Calafell J.M., Estop A.M., Egozcue J., Effect of the degree of maturation of mouse oocytes at fertilization: A source of chromosome imbalance, Gamete Res, 24, pp. 205-218, (1989)

[2]

Fraser L.R., Maudlin I., Analysis of aneuploidy in first cleavage mouse embryos fertilized in vitro and in vivo. Environ, Health Perspect, 31, pp. 141-149, (1979)

[3]

Janny L., Benkhalifa M., Pouly J.L., Vye P., Canis M., Geneix A., Malet, Boucher P.D., La condensation prématurée des chromosomes: Facteur frequent d’échec en fécondation in vitro? Contracept. Fertil, Sexual, 18, pp. 536-537, (1990)

[4]

Ma, Kalousek S., Zouves D.K., Youen C.B.H., Gomel V., Moon Y.S., Chromosome analysis of human oocytes failing to fertilize in vitro. Fertil, Steril, 51, pp. 992-997, (1989)

[5]

Macas E., Floersheim Y., Hotz E., Imthum B., Keller P.J., Walt H., Abnormal chromosomal arrangements in human oocytes. Hum, Reprod, 5, pp. 703-707, (1990)

[6]

Minshull J., Cyclin and MPF: Driving mitosis, Bioessays, 11, pp. 149-151, (1989)

[7]

Murray A.W., Kirschner M.W., Cyclin synthesis drives the early embryonic cell cycle, Nature, 33, pp. 275-280, (1989)

[8]

Papadopoulos G., Templeton A.A., Fisk N., Randall J., The frequency of chromosome anomalies in human preimplantation embryos after in-vitro fertilization. Hum, Reprod, 4, pp. 91-98, (1989)

[9]

Pieters M.H.E.C., Geraedts J.P.M., Dumoulin, Evers J., Bras M., Komips F.H.A.C., Menheere P.P.C.A., Cytogenetic analysis of in vitro fertilization (IVF) failures. Hum, Genet, 81, pp. 367-370, (1989)

[10]

Plachot M., De Grouchy J., Junca A., Mandelbaum J., Turleau C., Couillin P., Cohen J., Salat-Baroux J., From oocyte to embryo: A model, deduced from in vitro fertilization, for natural selection against chromosome abnormalities, Ann. Genet, 30, pp. 22-32, (1987)

← 1 2 3 →