THE HENS FERTILE EGG SCREENING-TEST (HEST) - A COMPARISON BETWEEN THE ACUTE TOXICITY FOR CHICK-EMBRYOS AND RODENTS OF 20 DRUGS

The possibilities of using developing chick embryos for evaluating drug activities and toxicities were studied by determining LD50 values for 20 drugs with 14 different pharmacological activities. Fifteen-day old chick embryos received drugs through the air cell and deaths were measured at 48 hr after the treatments. The LD50 values were determined and compared to the i.v., i,p., s.c. and p.o. values from mice listed in the Registry of Toxic Effects of Chemical Substance. The systemic toxicity of 15-day-old chick embryos to drugs were similar to those of mice with the following exceptions. The chick embryos seemed to be more sensitive than mice to antineoplastic or antibiotic agents such as actinomycin D and doxorubicin, whereas, LD50 values of cholinergic and cholinergic blocking drugs by this method were 10 to 20 fold of LD50 (i.v.) of mice. These observations are important for applying the hen's fertile screening test (HEST) to the determination of drug activities other than that of embryo toxicity or teratogenic activity.