IMMUNOELECTRON MICROSCOPIC DETECTION OF THE HEPATITIS-B VIRUS MAJOR SURFACE PROTEIN IN DILATED PERINUCLEAR MEMBRANES OF YEAST-CELLS

被引：16

作者：

BIEMANS, R ^{[1
]}

THINES, D ^{[1
]}

PETREPARENT, B ^{[1
]}

DEWILDE, M ^{[1
]}

RUTGERS, T ^{[1
]}

CABEZON, T ^{[1
]}

机构：

[1] CATHOLIC UNIV LOUVAIN,CELL BIOL LAB,B-1348 LOUVAIN,BELGIUM

来源：

DNA AND CELL BIOLOGY | 1992年 / 11卷 / 08期

关键词：

D O I：

10.1089/dna.1992.11.621

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The major surface protein of hepatitis B virus produced in Saccharomyces cerevisiae can be recovered from cell lysates in the form of 22-mm lipoprotein particles. Immunoelectron microscopy was applied to investigate site and time of particle assembly. Thin sections of yeast cells revealed that production of the S protein provoked a dilation of the endoplasmic reticulum. Dilated areas were specifically labeled with a polyclonal antibody raised against glutaraldehyde-treated yeast-derived HBsAg particles. In contrast to previous postulates of particle formation during cell lysis and extract preparation, these results suggest that particle formation in yeast occurs in the endoplasmic reticulum and that transport of particles along the secretion pathway is blocked.

引用

页码：621 / 626

页数：6

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