SIMPLE METHOD FOR EXTRACTING PLASMID DNA FROM LACTIC-ACID BACTERIA

被引：13

作者：

FRERE, J

机构：

[1] Irba, Laboratoire de Génétique Microbienne, Université de Caen, Caen

来源：

LETTERS IN APPLIED MICROBIOLOGY | 1994年 / 18卷 / 04期

关键词：

D O I：

10.1111/j.1472-765X.1994.tb00853.x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Rapid screening and large-scale plasmid DNA isolation procedures are described for lactic acid bacteria, using glass beads to break cells. The rapid screening procedure allows one to obtain plasmid DNA pellets in less than 1 h. This method has been successfully tested on various bacteria from the genera Lactococcus, Leuconostoc, Lactobacillus, Pediococcus, Streptococcus, Enterococcus and Propionibacterium. This procedure yields plasmid DNA with minor chromosomal and plasmid DNA-degraded form contaminations.

引用

页码：227 / 229

页数：3

共 8 条

[1] SIMPLE AND RAPID METHOD FOR ISOLATING LARGE PLASMID DNA FROM LACTIC STREPTOCOCCI [J].