SURVIVAL OF HUMAN OOCYTES CRYOPRESERVED WITH OR WITHOUT THE CUMULUS IN 1,2-PROPANEDIOL

被引：61

作者：

IMOEDEMHE, DG

SIGUE, AB

机构：

[1] Human Reproductive Biology Unit, Soliman Fakeeh Hospital, Jeddah, 21461

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1992年 / 9卷 / 04期

关键词：

CUMULUS; OOCYTE; CRYOPRESERVATION; PRECOOLED; 1,2-PROPANEDIOL;

D O I：

10.1007/BF01203954

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Background: Although cryopreservation of human preembryos has been carried out with success, the cryostorage of oocytes, which pose fewer controversial moral, ethical, and legal problems has been much less successful. Various attempts to cryopreserve human oocytes have been mostly unsuccessful and the search for an optimal protocol for oocyte cryopreservation remains elusive. We therefore undertook this study to determine the effect of oocyte cryostorage in 1,2-propanediol. Method: Mature human oocytes with or without their cumuli were cryopreserved in precooled 1,2-propanediol, then thawed and inseminated with sperms for in vitro fertilization. The outcome of insemination and subsequent embryonic development were also recorded and compared. Results: Postthaw cryosurvival rate was significantly better when cryostorage was carried out with the oocyte cumulus intact as compared to those oocytes denuded of their cumuli (54 versus 27%, respectively; P < 0.05). Eight (44%) of 18 surviving postthaw oocytes with intact cumuli were fertilized normally, with cleavage in six, as compared to two (25%) and one, respectively, of those denuded of their cumulus prior to cryostorage. Development to the blastocyst stage was achieved in three embryos derived from oocytes with an intact cumulus at cryostorage. Conclusion: We conclude that 1,2-propanediol can be used with success in oocyte cryopreservation, although the issue of parthenogenecity is still to be resolved. Oocyte's with intact cumulus survive cryostorage better than those without it.

引用

页码：323 / 327

页数：5

共 25 条

[11]

Kola I., Kirby C., Shaw J., Davey A., Trounson A., Vitrification of mouse oocytes results in aneuploid zygotes and malformed fetuses, Teratology, 38, (1988)

[12]

Sathananthan A.H., Trounson A., Freeman L., Brady T., The effect of cooling human oocytes, Hum Reprod, 3, pp. 968-977, (1988)

[13]

Al-Hasani S., Tolkdorf A., Diedrich K., Van der Ven H., Krebs D., Successful in vitro fertilization of frozen thawed rabbit oocytes, Hum Reprod, 1, pp. 309-312, (1986)

[14]

Glenister P.H., Wood M.J., Kirby C., Whittingham D.G., Incidence of chromosome anomalies in first cleavage mouse embryos obtained from frozen thawed oocytes fertilized in vitro, Gamete Res, 16, pp. 205-216, (1987)

[15]

Vincent C., Pickering S.J., Johnson M.H., The zona hardening effect of dimethyl sulfoxide requires the presence of an oocyte and is associated with reduction in the number of cortical granules present, J Reprod Fert, 89, pp. 253-259, (1990)

[16]

Vincent C., Pickering S.J., Johnson M.H., Quick S.J., Dimethyl sulfoxide affects the organization of microfilaments in the mouse oocyte, Mol Reprod Dev, 26, pp. 227-235, (1990)

[17]

Johnson M.H., Pickering S.J., The effect of dimethylsulfoxide on the microtubular system of the mouse oocyte, Development, 100, pp. 313-324, (1987)

[18]

Van der Eist J., Van der Abbeel E., Jacobs R., Wisse E., Van Steirteghem A., Effect of 1,2-propanediol and dimethyl sulfoxide on the meiotic spindle of mouse oocyte, Hum Reprod, 3, pp. 960-967, (1988)

[19]

Johnson M.H., The effect of fertilization of exposure of mouse oocytes to dimethyl sulfoxide: An optimal protocol, J Vitro Fert Embryo Transfer, 6, pp. 168-175, (1989)

[20]

Pickering S.J., Braude P.R., Johnson M.H., Cryoprotection of human oocytes: Inappropriate exposure to DMSO reduces fertilization rates, Hum Reprod, 6, pp. 142-143, (1991)

← 1 2 3 →