ENHANCED INTRACELLULAR DELIVERY OF DOXORUBICIN BY SCAVENGER RECEPTOR-MEDIATED ENDOCYTOSIS FOR PREFERENTIAL KILLING OF HISTIOCYTIC LYMPHOMA-CELLS IN CULTURE

被引：10

作者：

BASU, S ^{[1
]}

MUKHOPADHYAY, B ^{[1
]}

BASU, SK ^{[1
]}

MUKHOPADHYAY, A ^{[1
]}

机构：

[1] NATL INST IMMUNOL,NEW DELHI 110067,INDIA

来源：

FEBS LETTERS | 1994年 / 342卷 / 03期

关键词：

DRUG DELIVERY; SCAVENGER RECEPTOR; ENDOCYTOSIS; HISTIOCYTIC MALIGNANCY; CANCER CHEMOTHERAPY;

D O I：

10.1016/0014-5793(94)80511-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A conjugate of the antineoplastic drug doxorubicin (DXR) with maleylated bovine serum albumin (MBSA) was taken up by a human histiocytic lymphoma cell line (U937) through the high efficiency process of scavenger receptor-mediated endocytosis resulting in a sixfold higher intracellular concentration of the drug compared to that obtained when the free drug was administered. Compared to the free drug, the drug conjugate showed significantly higher cytotoxicity towards U937 cells presumably because of intracellular availability of a pharmacologically active form of the drug. The intracellular product released after lysosomal degradation of the drug conjugate was chromatographically identical to free DXR. These findings merit serious consideration in the development of new chemotherapeutic agents for the treatment of histiocytic malignancies.

引用

页码：249 / 254

页数：6

共 21 条

[1]

BIJSTERBOSCH M K, 1990, Advanced Drug Delivery Reviews, V5, P231, DOI 10.1016/0169-409X(90)90018-N

[2]

BUZDAR AU, 1985, CANCER, V55, P2761, DOI 10.1002/1097-0142(19850615)55:12<2761::AID-CNCR2820551206>3.0.CO

[3]

2-P

[4] SELECTIVE DELIVERY OF DRUGS TO MACROPHAGES THROUGH A HIGHLY SPECIFIC RECEPTOR - AN EFFICIENT CHEMOTHERAPEUTIC APPROACH AGAINST LEISHMANIASIS [J].

CHAUDHURI, G ;

MUKHOPADHYAY, A ;

BASU, SK .

BIOCHEMICAL PHARMACOLOGY, 1989, 38 (18) :2995-3002

[5]

CLINE MJ, 1980, CANCER TREATMENT, P952

[6]

FORSSEN EA, 1983, CANCER RES, V43, P546

[7]

GHOSE TI, 1983, METHOD ENZYMOL, V93, P280

[8]

GLAZER AN, 1975, CHEM MODIFICATIONS P, P79

[9]

GOLDSTEIN JL, 1983, METHOD ENZYMOL, V98, P241

[10] BINDING-SITE ON MACROPHAGES THAT MEDIATES UPTAKE AND DEGRADATION OF ACETYLATED LOW-DENSITY LIPOPROTEIN, PRODUCING MASSIVE CHOLESTEROL DEPOSITION [J].

GOLDSTEIN, JL ;

HO, YK ;

BASU, SK ;

BROWN, MS .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1979, 76 (01) :333-337

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