DETECTION OF EPSTEIN-BARR-VIRUS GENOME IN SINONASAL UNDIFFERENTIATED CARCINOMA BY USE OF IN-SITU HYBRIDIZATION

被引：27

作者：

GALLO, O ^{[1
]}

DILOLLO, S ^{[1
]}

GRAZIANI, P ^{[1
]}

GALLINA, E ^{[1
]}

BARONI, G ^{[1
]}

机构：

[1] UNIV FLORENCE,INST PATHOL,I-50134 FLORENCE,ITALY

来源：

OTOLARYNGOLOGY-HEAD AND NECK SURGERY | 1995年 / 112卷 / 06期

关键词：

D O I：

10.1016/S0194-5998(95)70172-9

中图分类号：

R76 [耳鼻咽喉科学];

学科分类号：

100213 ;

摘要：

Associations between Epstein-Barr virus and undifferentiated carcinomas of nasopharynx, parotid gland, and thymus have recently. been reported. Epstein-Barr virus has also been associated with malignant lymphoma of the nose and paranasal sinuses. These findings raise the possibility that Epstein-Barr virus may additionally be linked to undifferentiated carcinoma of the nose and paranasal sinuses (SNUG), an uncommon but distinctive and highly aggressive neoplasm. Histologically, SNUG consists of small and medium cells, the precise characterization of which often requires immunocytochemical analysis. This study investigates the presence of DNA sequences of Epstein-Barr virus in biopsy specimens of 13 cases of SNUG that were defined immunocytochemically by use of previously reported criteria. In situ hybridization was used to detect Epstein-Barr virus genome in different cell types in routinely-processed, paraffin-embedded tissues. Epstein-Barr virus-specific DNA sequences were detected in tumor cells of SNUG specimens from 5 of the 13 cases examined. No correlation was found between positive hybridization and primary tumor site, morphologic subtype, or disease course. Epstein-Barr virus DNA was detected in 38% (5 of 13) of the SNUG samples analyzed. This finding suggests that this virus may play a role in the pathogenesis of this rare neoplasm.

引用

页码：659 / 664

页数：6

共 23 条

[1] Thorley-Lawson D.A., Basic virologic aspects of Epstein-Barr virus infection, Semin Hematol, 25, pp. 247-260, (1988)
[2] Miller G., Epstein Barr virus: biology, pathogenesis, and medical aspects, Virology, pp. 1921-1958, (1990)
[3] Wolf H., Hus M., Wilmes E., Persistence of Epstein-Barr virus in the parotid gland, J Virol, 51, pp. 795-798, (1984)
[4] Sixbey J., Nedrud J.G., Raab-Traub N., Hanes R.A., Pagano J.S., Detection of Epstein-Barr virus DNA and RNA in human pharyngeal epithelial cells, N Engl J Med, 310, pp. 1225-1230, (1984)
[5] Weiss L.M., Movahed L.A., Warnke R.A., Sklar J., Detection of EBV genomes in Reed-Sternberg cells of Hodgkin's disease, N Engl J Med, 320, pp. 502-506, (1989)
[6] Schulte-Holthausen H.S., Klein G., Et al., EBV DNA in biopsies of Burkitt tumours and anaplastic carcinomas of the nasopharynx, Nature, 228, pp. 1056-1058, (1970)
[7] Ho F.C.S., Srivastava G., Loke S.L., Ng R.P., Khoo R.K.K., Presence of clonal Epstein-Barr virus in nasal lymphomas of B and T cell type, Semin Hematol, 8, pp. 271-281, (1990)
[8] Weiss L.M., Gaffey M.J., Chen Y.Y., Frierson H.F., Frequency of Epstein-Barr viral DNA in Western sinonasal and Waldeyer's ring non-Hodgkin's lymphomas, Am J Surg Pathol, 16, pp. 156-162, (1992)
[9] Ott G., Ott M., Feller A.C., Seidl S., Muller-Hermelink H.K., Prevalence of Epstein-Barr virus DNA in different T-cell lymphoma entities in a European population, Int J Cancer, 51, pp. 562-567, (1992)
[10] Weiss L.M., Movahed L.A., Butler A.E., Analysis of lymphoepithelioma and lymphoepithelioma-like carcinomas for Epstein-Barr virus genomes by in situ hybridization, Am J Surg Pathol, 11, pp. 625-631, (1989)

← 1 2 3 →