SOME PROPERTIES OF GAMMA-TOCOPHEROL METHYLTRANSFERASE SOLUBILIZED FROM SPINACH-CHLOROPLASTS

被引：10

作者：

ISHIKO, H

SHIGEOKA, S

NAKANO, Y

MITSUNAGA, T

机构：

[1] KINKI UNIV,DEPT FOOD & NUTR,NARA 631,JAPAN

[2] UNIV OSAKA PREFECTURE,DEPT AGR CHEM,SAKAI,OSAKA 591,JAPAN

来源：

PHYTOCHEMISTRY | 1992年 / 31卷 / 05期

关键词：

SPINACIA-OLERACEA; CHENOPODIACEAE; SPINACH; CHLOROPLASTS; METHYLTRANSFERASE; GAMMA-TOCOPHEROL;

D O I：

10.1016/0031-9422(92)83094-F

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Gamma-Tocopherol methyltransferase occurs in the chloroplast fraction of spinach leaves. Its specific activity with gamma-tocopherol and S-adenosyl-L-methionine was 3.91 nmol hr-1 mg-1 protein. The enzyme was effectively solubilized by 6 mM sodium deoxycholate from the membrane fraction of chloroplasts. The activity was maximum at pH 7.5 and 35-degrees. Gamma-Tocopherol was preferred to beta-tocopherol (25:7). The K(m) value for S-adenosyl-L-methionine as methyl donor was 9.1-mu-M.

引用

页码：1499 / 1500

页数：2

共 13 条

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[2]

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