ALPHA-9 - AN ACETYLCHOLINE-RECEPTOR WITH NOVEL PHARMACOLOGICAL PROPERTIES EXPRESSED IN RAT COCHLEAR HAIR-CELLS

被引：747

作者：

ELGOYHEN, AB ^{[1
]}

JOHNSON, DS ^{[1
]}

BOULTER, J ^{[1
]}

VETTER, DE ^{[1
]}

HEINEMANN, S ^{[1
]}

机构：

[1] SALK INST BIOL STUDIES, LA JOLLA, CA 92037 USA

来源：

CELL | 1994年 / 79卷 / 04期

关键词：

D O I：

10.1016/0092-8674(94)90555-X

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We report the isolation and funational characterization of a member of the nicotinic acetylcholine receptor subunit gene family, alpha 9. Xenopus oocytes injected with alpha 9 cRNA express a homomeric receptor-channel complex that is activated by acetylcholine. The alpha 9 receptor displays an unusual mixed nicotinic-muscarinic pharmacological profile. The unique properties of the alpha 9 receptor-channel complex closely match those described for the cholinergic receptor present in vertebrate cochlear hair cells. In situ hybridization studies reveal a restricted pattern of alpha 9 gene expression that includes the outer hair cells of the rat cochlea. Our results suggest that the alpha 9 receptor is involved in the cholinergic efferent innervation of cochlear hair cells and thus may modulate the encoding of auditory stimuli.

引用

页码：705 / 715

页数：11

共 50 条

[1] ELECTROPHYSIOLOGY OF A CHICK NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTOR EXPRESSED IN XENOPUS OOCYTES AFTER CDNA INJECTION
BALLIVET, M
NEF, P
COUTURIER, S
RUNGGER, D
BADER, CR
BERTRAND, D
COOPER, E
[J]. NEURON, 1988, 1 (09) : 847 - 852
[2] IDENTIFICATION OF A FAMILY OF MUSCARINIC ACETYLCHOLINE-RECEPTOR GENES
BONNER, TI
BUCKLEY, NJ
YOUNG, AC
BRANN, MR
[J]. SCIENCE, 1987, 237 (4814) : 527 - 532
[3] FUNCTIONAL EXPRESSION OF 2 NEURONAL NICOTINIC ACETYLCHOLINE-RECEPTORS FROM CDNA CLONES IDENTIFIES A GENE FAMILY
BOULTER, J
CONNOLLY, J
DENERIS, E
GOLDMAN, D
HEINEMANN, S
PATRICK, J
[J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1987, 84 (21) : 7763 - 7767
[4] BOULTER J, 1990, J BIOL CHEM, V265, P4472
[5] THE ACTIVITY OF OLFACTORY RECEPTOR-CELLS IS AFFECTED BY ACETYLCHOLINE AND SUBSTANCE-P
BOUVET, JF
DELALEU, JC
HOLLEY, A
[J]. NEUROSCIENCE RESEARCH, 1988, 5 (03) : 214 - 223
[6] EFFERENT CONTROL OF COCHLEAR INNER HAIR CELL RESPONSES IN THE GUINEA-PIG
BROWN, MC
NUTTALL, AL
[J]. JOURNAL OF PHYSIOLOGY-LONDON, 1984, 354 (SEP): : 625 - 646
[7] BUONANNO A, 1989, J BIOL CHEM, V264, P7611
[8] CHANG C. C., 1963, ARCH INTERNATL PHARMACODYN THER, V144, P241
[9] CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2
[10] COUTURIER S, 1990, J BIOL CHEM, V265, P17560

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