MATRIX DESIGN FOR MATRIX-ASSISTED LASER-DESORPTION IONIZATION - SENSITIVE DETERMINATION OF PAH-DNA ADDUCTS

被引：14

作者：

GEORGE, M

WELLEMANS, JMY

CERNY, RL

GROSS, ML

LI, K

CAVALIERI, EL

机构：

[1] UNIV NEBRASKA,DEPT CHEM,MIDWEST CTR MASS SPECTROMETRY,LINCOLN,NE 68588

[2] UNIV NEBRASKA,MED CTR,EPPLEY INST RES CANC & ALLIED DIS,OMAHA,NE

来源：

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY | 1994年 / 5卷 / 11期

基金：

美国国家卫生研究院;

关键词：

D O I：

10.1016/1044-0305(94)80021-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Two matrices, 4-phenyl-alpha-cyanocinnamic acid (PCC) and 4-benzyloxy-alpha-cyanocinnamic acid (BCC), were identified for the determination of polycyclic aromatic hydrocarbon (PAH) adducts of DNA bases by matrix-assisted laser desorption ionization. These matrices were designed based on the concept that the matrix and the analyte should have structural similarities. PCC is a good matrix for the desorption of not only PAH-modified DNA bases, but also PAHs themselves and their metabolites. Detections can be made at the femtomolar level.

引用

收藏

页码：1021 / 1025

页数：5

相关论文

共 29 条

[1] MASS-SPECTROMETRY OF SYNTHETIC-POLYMERS BY UV MATRIX-ASSISTED LASER DESORPTION IONIZATION [J].

BAHR, U ;

DEPPE, A ;

KARAS, M ;

HILLENKAMP, F ;

GIESSMANN, U .

ANALYTICAL CHEMISTRY, 1992, 64 (22) :2866-2869

[2] BINDING OF BENZO[A]PYRENE TO DNA BY CYTOCHROME-P-450 CATALYZED ONE-ELECTRON OXIDATION IN RAT-LIVER MICROSOMES AND NUCLEI [J].

CAVALIERI, EL ;

ROGAN, EG ;

DEVANESAN, PD ;

CREMONESI, P ;

CERNY, RL ;

GROSS, ML ;

BODELL, WJ .

BIOCHEMISTRY, 1990, 29 (20) :4820-4827

[3] PROTEIN LADDER SEQUENCING [J].

CHAIT, BT ;

WANG, R ;

BEAVIS, RC ;

KENT, SBH .

SCIENCE, 1993, 262 (5130) :89-92

[4] MASS-SPECTROMETRY FOR THE ANALYSIS OF CARCINOGEN-DNA ADDUCTS [J].

CHIARELLI, MP ;

LAY, JO .

MASS SPECTROMETRY REVIEWS, 1992, 11 (06) :447-493

[5] FENBUFEN, A NEW ANTI-INFLAMMATORY ANALGESIC - SYNTHESIS AND STRUCTURE-ACTIVITY-RELATIONSHIPS OF ANALOGS [J].

CHILD, RG ;

OSTERBERG, AC ;

SLOBODA, AE ;

TOMCUFCIK, AS .

JOURNAL OF PHARMACEUTICAL SCIENCES, 1977, 66 (04) :466-476

[6] MASS-SPECTROMETRIC TECHNIQUES IN NUCLEIC-ACID RESEARCH [J].

CRAIN, PF .

MASS SPECTROMETRY REVIEWS, 1990, 9 (05) :505-554

[7] THE ANALYSIS OF WATER-SOLUBLE POLYMERS BY MATRIX-ASSISTED LASER DESORPTION TIME-OF-FLIGHT MASS-SPECTROMETRY [J].

DANIS, PO ;

KARR, DE ;

MAYER, F ;

HOLLE, A ;

WATSON, CH .

ORGANIC MASS SPECTROMETRY, 1992, 27 (07) :843-846

[8] A FACILE SAMPLE PREPARATION FOR THE ANALYSIS OF SYNTHETIC ORGANIC POLYMERS BY MATRIX-ASSISTED LASER-DESORPTION IONIZATION [J].

DANIS, PO ;

KARR, DE .

ORGANIC MASS SPECTROMETRY, 1993, 28 (08) :923-925

[9] STRUCTURAL CHARACTERIZATION OF NORMAL AND MODIFIED OLIGONUCLEOTIDES BY MATRIX-ASSISTED LASER DESORPTION FOURIER-TRANSFORM MASS-SPECTROMETRY [J].

HETTICH, R ;

BUCHANAN, M .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1991, 2 (05) :402-412

[10] MATRIX-ASSISTED LASER DESORPTION IONIZATION MASS-SPECTROMETRY WITH 2-(4-HYDROXYPHENYLAZO)BENZOIC ACID MATRIX [J].

JUHASZ, P ;

COSTELLO, CE ;

BIEMANN, K .

JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY, 1993, 4 (05) :399-409