IDENTIFICATION AND CHARACTERIZATION OF ALLERGENS RELATED TO BET-V-I, THE MAJOR BIRCH POLLEN ALLERGEN, IN APPLE, CHERRY, CELERY AND CARROT BY 2-DIMENSIONAL IMMUNOBLOTTING AND N-TERMINAL MICROSEQUENCING

被引：43

作者：

SCHONING, B ^{[1
]}

VIETHS, S ^{[1
]}

PETERSEN, A ^{[1
]}

BALTES, W ^{[1
]}

机构：

[1] FORSCHUNGSINST BORSTEL, DIV ALLERGOL, BORSTEL, GERMANY

来源：

JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE | 1995年 / 67卷 / 04期

关键词：

APPLE ALLERGENS; BIRCH POLLEN ALLERGENS; CARROT ALLERGENS; CELERY ALLERGENS; CHERRY ALLERGENS; CROSS-REACTIVITY; IMMUNOBLOTTING; N-TERMINAL MICROSEQUENCING; 2-DIMENSIONAL ELECTROPHORESIS;

D O I：

10.1002/jsfa.2740670403

中图分类号：

S [农业科学];

学科分类号：

09 ;

摘要：

Low temperature extracts taken from Granny Smith and Braeburn apples, cherries, celery and carrots were separated by two-dimensional electrophoresis. Bet nu I, the major birch pollen allergen, related allergens in apple, cherry, celery and carrot extracts were detected by means of two-dimensional immunoblotting with patients' sera containing IgE antibodies specific to Bet nu I, a rabbit polyclonal antiserum raised against Bet nu I, and two Bet nu I specific mouse monoclonal antibodies. The major cross-reacting allergen spots were observed with molecular weights/isoelectric points of 18.0 kDa/pI 5.5 for apple (Granny Smith and Braeburn) and 18.0 kDa/pI 5.8 for cherry, 15.5 kDa/pI 4.4-4.6 for celery and 16.0 kDa/pI 4.4 for carrot extract. Additional antibody reactivities with certain isoprotein spots were observed, which may indicate the presence of Bet nu I-related epitopes on these proteins. Based on the first 15 N-terminal amino acid residues, the major allergen spots revealed 53% sequence identity between Bet nu I and the Granny Smith apple allergen, 50% between Braeburn apple allergen and Bet nu I, 67% between Bet nu I and the cherry allergen, and 40 and 28% for celery and carrot, respectively. Furthermore, the N-terminal sequences showed identities ranging from 40% (apple/cherry) to 66%(celery) with PcPR l-l, a pathogenesis-related protein in parsley.

引用

页码：431 / 440

页数：10

共 36 条

[1] IMMUNOGLOBULIN-E ANTIBODIES THAT CROSSREACT WITH VEGETABLE FOODS, POLLEN, AND HYMENOPTERA VENOM [J].

AALBERSE, RC ;

KOSHTE, V ;

CLEMENS, JGJ .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1981, 68 (05) :356-364

[2]

AAS K, 1988, FOOD ALLERGY, P5

[3] EXTRACTION AND PROPERTIES OF APPLE ALLERGENS [J].

BJORKSTEN, F ;

HALMEPURO, L ;

HANNUKSELA, M ;

LAHTI, A .

ALLERGY, 1980, 35 (08) :671-677

[4] THE GENE CODING FOR THE MAJOR BIRCH POLLEN ALLERGEN BETVL, IS HIGHLY HOMOLOGOUS TO A PEA DISEASE RESISTANCE RESPONSE GENE [J].

BREITENEDER, H ;

PETTENBURGER, K ;

BITO, A ;

VALENTA, R ;

KRAFT, D ;

RUMPOLD, H ;

SCHEINER, O ;

BREITENBACH, M .

EMBO JOURNAL, 1989, 8 (07) :1935-1938

[5] CROSS-REACTIVITY AMONG BIRCH POLLEN, VEGETABLES AND FRUITS AS DETECTED BY IGE ANTIBODIES IS DUE TO AT LEAST 3 DISTINCT CROSS-REACTIVE STRUCTURES [J].

CALKHOVEN, PG ;

AALBERS, M ;

KOSHTE, VL ;

POS, O ;

OEI, HD ;

AALBERSE, RC .

ALLERGY, 1987, 42 (05) :382-390

[6]

DREBORG S, 1988, ANN ALLERGY, V61, P41

[7] COMMON EPITOPES OF BIRCH POLLEN AND APPLES - STUDIES BY WESTERN AND NORTHERN BLOT [J].

EBNER, C ;

BIRKNER, T ;

VALENTA, R ;

RUMPOLD, H ;

BREITENBACH, M ;

SCHEINER, O ;

KRAFT, D .

JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY, 1991, 88 (04) :588-594

[8] FOOD HYPERSENSITIVITY IN PATIENTS WITH POLLEN ALLERGY [J].

ERIKSSON, NE ;

FORMGREN, H ;

SVENONIUS, E .

ALLERGY, 1982, 37 (06) :437-443

[9]

Gell PGH, 1963, CLIN ASPECTS IMMUNOL

[10] IMMUNOLOGICAL INVESTIGATION OF POSSIBLE STRUCTURAL SIMILARITIES BETWEEN POLLEN ANTIGENS AND ANTIGENS IN APPLE, CARROT AND CELERY TUBER [J].

HALMEPURO, L ;

LOWENSTEIN, H .

ALLERGY, 1985, 40 (04) :264-272

← 1 2 3 4 →