QUANTITATIVE-ANALYSIS OF DNA ABERRATIONS AMPLIFIED BY COMPETITIVE POLYMERASE CHAIN-REACTION USING CAPILLARY ELECTROPHORESIS

被引：27

作者：

KUYPERS, AWHM

MEIJERINK, JPP

SMETSERS, TFCM

LINSSEN, PCM

MENSINK, EJBM

机构：

[1] UNIV NIJMEGEN HOSP, DEPT INTERNAL MED, DIV HEMATOL, 6525 GA NIJMEGEN, NETHERLANDS

[2] UNIV NIJMEGEN HOSP, CENT HEMATOL LAB, 6525 GA NIJMEGEN, NETHERLANDS

来源：

JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES | 1994年 / 660卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00285-1

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We compared the use of capillary electrophoresis (CE) in a polymer network with the use of slab gel electrophoresis for the quantitative analysis of polymerase chain reaction (PCR)-amplified DNA samples. We quantified residual lymphoma cells carrying a translocation between chromosomes 14 and 18, in consecutive patient peripheral blood samples that were amplified by competitive PCR. For CE analysis we used a 4% linear polyacrylamide network. Results show that the calculated number of translocations in patient samples using both analyses were comparable. We conclude that CE is a sensitive, non-radioactive, fast and accurate method for quantitation of competitive PCR products.

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页码：271 / 277

页数：7

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