SPECIFIC EXCISION OF INSERTED DNA SEGMENT FROM HYBRID PLASMIDS CONSTRUCTED BY POLY(DA).POLY(DT) METHOD

被引：49

作者：

HOFSTETTER, H ^{[1
]}

SCHAMBOCK, A ^{[1
]}

VANDENBERG, J ^{[1
]}

WEISSMANN, C ^{[1
]}

机构：

[1] UNIV ZURICH, INST MOLEKULARBIOL, CH-8093 ZURICH, SWITZERLAND

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1976年 / 454卷 / 03期

关键词：

D O I：

10.1016/0005-2787(76)90286-0

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The Escherichia coli hybrid plasmid P.beta.G, consisting of plasmid PMB9 DNA with an insert of rabbit globin DNA (about 600 base pairs) flanked by poly(dA).cntdot.poly(dT) regions was cleaved into 2 fragments by endonuclease S1 under conditions of partial denaturation. Only the smaller fragment (575 base pairs) contained globin-specific sequences, showing that excision had occurred in the A.cntdot.T-rich regions. This method of cleavage provides a useful procedure for assessing the length of inserts in hybrid plasmids prepared by the poly(dA).cntdot.poly(dT) tail method, and allows the preparative recovery of the insert.

引用

页码：587 / 591

页数：5

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