A GC-MS METHOD FOR DETERMINATION OF DELTA-9-TETRAHYDROCANNABINOL-11-OIC ACID IN HUMAN URINE

被引：8

作者：

YAMAMOTO, I

WATANABE, K

NARIMATSU, S

MATSUNAGA, T

KIJIMA, T

HIRAIWA, K

YOSHIMURA, H

机构：

[1] Department of Hygienic Chemistry, Faculty of Pharmaceutical Sciences, Hokuriku University, Kanazawa 920-11

[2] Department of Legal Medicine, Fukushima Medical College, Fukushima 960, 5-75, Sugizuma-cho

[3] Department of Hygienic and Forensic Chemistry, Faculty of Pharmaceutical Sciences, Kyushu University, Fukuoka 812, 3-1-1, Maidashi, Higashi-ku

来源：

EISEI KAGAKU-JAPANESE JOURNAL OF TOXICOLOGY AND ENVIRONMENTAL HEALTH | 1990年 / 36卷 / 02期

关键词：

5'-nor-Δ8-tetrahydrocannabinol-4'-oic acid; Fast Blue BB salt; GC-MS; human urine; TLC; Δ9-tetrahydrocannabinol; Δ9-tetrahydrocannabinol-11-oic acid;

D O I：

10.1248/jhs1956.36.149

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

The GC-MS and TLC methods for the determination and identification of Δ9-tetrahydrocannabinol-11-oic acid, a major urinary metabolite of Δ9-tetrahydrocannabinol which is one of main constituents of marihuana, were established. Δ9-Tetrahydrocannabinol-11-oic acid was detected on TLC by using Fast Blue BB salt as a color reagent. The metabolite was determined as trimethylsilyl derivative of its methyl ester by using electron impact ionization GC-MS and 5'-nor-Δ8-tetrahydrocannabinol-4'-oic acid as an internal standard. The assay provides excellent sensitivity, reproducibility and specificity with detection limit of 1 ng/ml human urine. © 1990, The Pharmaceutical Society of Japan. All rights reserved.

引用

页码：149 / 152

页数：4

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