SIMULTANEOUS ISOCRATIC HPLC SEPARATION OF THE DIASTEREOISOMERS OF CAFFEIC, FERULIC, AND ISOFERRULIC ACIDS AND RELATED COUMARINS

被引：7

作者：

BORGES, MFM

ROLEIRA, FMF

PINTO, MMM

机构：

[1] Laboratorio de Química Orgânica, Faculdade de Farmácia do Porto, Rua Anibal Cunha

来源：

JOURNAL OF LIQUID CHROMATOGRAPHY | 1993年 / 16卷 / 01期

关键词：

D O I：

10.1080/10826079308020903

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A general procedure for the simultaneous analysis of phenolic compounds by a reversed-phase high performance liquid chromatography (RP-HPLC) is described. The series of compounds embraces the cis/trans isomers of caffeic, ferulic and isoferulic acids and the coumarins - esculetin, scopoletin and isoscopoletin. In order to establish the experimental conditions, leading to an optimal resolution, several test mixtures were analysed. HPLC separation was carried out on a reversed-phase column ( Lichrosorb RP-8, 5 mum), using aqueous tetrahydrofuran as a mobile phase.

引用

页码：149 / 160

页数：12

共 9 条

[1]

BARTON D, 1979, COMPRHENSIVE ORGANIC

[2] ISOCRATIC HPLC SEPARATION OF SCOPOLETIN AND CIS/TRANS ISOMERS OF FERULIC ACID AS WELL AS ISOSCOPOLETIN AND CIS-TRANS ISOMERS OF ISOFERULIC ACID [J].