POSTANOXIC OXIDATIVE INJURY IN RAT HEPATOCYTES - LACTATE-DEPENDENT PROTECTION AGAINST TERT-BUTYLHYDROPEROXIDE

被引:26
作者
KOWALSKI, DP
AW, TY
PARK, Y
JONES, DP
机构
[1] EMORY UNIV,DEPT BIOCHEM,ATLANTA,GA 30322
[2] EMORY UNIV,DEPT PEDIAT,ATLANTA,GA 30322
[3] EMORY UNIV,WINSHIP CANC CTR,ATLANTA,GA 30322
[4] LOUISIANA STATE UNIV,MED CTR,DEPT PHYSIOL & BIOPHYS,SHREVEPORT,LA 71130
关键词
PEROXIDES; REOXYGENATION INJURY; ISCHEMIA; FREE RADICALS;
D O I
10.1016/0891-5849(92)90028-F
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Previous studies in this laboratory showed that hypoxia and anoxia enhance the susceptibility of hepatocytes to tert-butylhydroperoxide (TBH)-induced oxidative injury. 6 To determine whether preceding exposure to anoxia affects postanoxic sensitivity to oxidative injury, viability was studied in hepatocytes incubated under anoxic conditions followed by reoxygenation without or with tert-butylhydroperoxide addition. Results showed that a preceding exposure to 60 min of anoxia substantially increased the vulnerability of cells to injury by the oxidant. Because substantial tissue lactate can accumulate during anoxia, the effect of increased lactate on postanoxic injury due to TBH was determined. Results showed that added lactate protected in a concentration-dependent manner. The TBH elimination rate was stimulated by lactate, and the pyruvate production rate approached the rate of TBH elimination. Thus, lactate protects against postanoxic oxidative injury by supplying reducing equivalents for peroxide reduction. This suggests that lactate accumulation during ischemia may be beneficial and that supplementation with lactate could be considered as a means to protect against postischemic injury.
引用
收藏
页码:205 / 212
页数:8
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