LIDOCAINE, A LOCAL-ANESTHETIC, REVERSIBLY INHIBITS CYTOPLASMIC STREAMING IN VALLISNERIA MESOPHYLL-CELLS

被引:2
作者
GARCIASIERRA, F
FRIXIONE, E
机构
[1] INST POLITECN NACL,CTR INVEST & ESTUD AVANZADOS,DEPT BIOL CELLULAR,APDO POSTAL 14-740,MEXICO CITY 07000,DF,MEXICO
[2] INST POLITECN NACL,CTR INVEST & ESTUD AVANZADUS,DEPT FISIOL BIOFIS & NEUROCIENCIAS,MEXICO CITY,DF,MEXICO
关键词
CELL MOTILITY; LOCAL ANESTHETICS; PLANT CELLS; VALLISNERIA;
D O I
10.1007/BF01385014
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
Lidocaine, which like other local anesthetics is known to inhibit intracellular transport in animal cells, was tested for its effect on the rotational cytoplaSMiC streaming in the mesophyll cells of the aquatic plant Vallisneria. The drug caused reversible inhibition of cytoplasmic streaming in a dose dependent manner within the 2-20 mM range; higher concentrations resulted in permanent cessation of all cytoplasmic motion. Upon recovery following replacement of the normal bathing medium, cytoplasmic rotation was always resumed in the direction of the original movement exhibited by a given cell. The lidocaine effect was virtually independent of the ionic composition of the incubation medium, but it was markedly affected by the external pH; acidic conditions (pH 6) largely prevented the inhibition of streaming, whereas an alkaline environment (pH 8) accelerated both the onset of the effect and the recovery upon removal of the anesthetic. On the basis of these results and findings in other systems, it is suggested that lidocaine acts through interference with mechanisms that regulate cytoplasmic streaming, rather than with the motile apparatus or the supply of metabolic energy.
引用
收藏
页码:153 / 160
页数:8
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