EFFECT OF 2 ISOMERIC OCTADECENOIC ACIDS ON LIPID-METABOLISM AND GROWTH OF NOVIKOFF HEPATOMA-CELLS

被引：10

作者：

WENNERSTROM, DE ^{[1
]}

JENKIN, HM ^{[1
]}

机构：

[1] UNIV MINNESOTA HORMEL INST, AUSTIN, MN 55912 USA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1976年 / 431卷 / 03期

关键词：

D O I：

10.1016/0005-2760(76)90213-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The origin and metabolism of octadecenoic acid (18:1) was examined in intact Novikoff rat hepatoma cells by using labeled precursors and 2 isomeric octadecenoic acids which differed in their abilities to stimulate cell growth in serum free medium. The isomers (cis-6-18:1 and cis-9-18:1) were measured in the cellular lipid by ozonolysis and reduction of the ozonides. The results indicate that the 18:1 fatty acid accumulated in the cell lipid by uptake of the preformed acid from the medium. The cis-6-18:1 isomer was more extensively metabolized than the cis-9-18:1 isomer to 16:1 and 20:1 fatty acids by chain shortening and chain elongation. Both isomers inhibited de novo fatty acid synthesis from acetate by cells suspended in a serum-free medium. The isomers did not exert coordinate control of fatty acid and cholesterol biosynthesis in the Novikoff cells.

引用

页码：469 / 480

页数：12

共 26 条

[1] STUDIES ON LIPID AND FATTY-ACID COMPOSITION OF HUMAN HEPATOMA TISSUE [J].