S9-ACTIVATED AMES ASSAYS OF DIESEL-PARTICLE EXTRACTS - DETECTING INDIRECT-ACTING MUTAGENS IN SAMPLES THAT ARE DIRECT-ACTING

被引：17

作者：

BALL, JC ^{[1
]}

GREENE, B ^{[1
]}

YOUNG, WC ^{[1
]}

RICHERT, JFO ^{[1
]}

SALMEEN, IT ^{[1
]}

机构：

[1] FORD MOTOR CO,SCI RES LABS,ROOM 53083,POB 2053,DEARBORN,MI 48121

来源：

ENVIRONMENTAL SCIENCE & TECHNOLOGY | 1990年 / 24卷 / 06期

关键词：

D O I：

10.1021/es00076a016

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

We sought to detect S9-activated mutagens in dieselexhaust particle extracts by fractionating extracts using high-performance liquid chromatography (HPLC) and carrying out Ames assays (strains TA98 and TA100) with and without S9 mix on the eight fractions obtained thereby. S9 activation of the fraction containing unsubstituted and alkyl-substituted polycyclic aromatic hydrocarbons (PAH) was at the limits of detectability for strain TA100 and undetectable for TA98. There was only one fraction that showed S9 activation; it was 20-fold more active with S9 mix. Six other fractions were strongly direct-acting and showed no enhancement of activity with S9 mix. The S9-activated mutagens detected when concentrated in a chromatographic fraction could not be detected in unfractionated extracts because the direct-acting mutagenicity of the other components dominated. We were unable to identify the indirect-acting mutagens from GC-MS data. © 1990, American Chemical Society. All rights reserved.

引用

页码：890 / 894

页数：5

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