DIAGNOSIS OF EQUID HERPESVIRUSES-1 AND HERPESVIRUSES-4 BY POLYMERASE CHAIN-REACTION

被引：63

作者：

SHARMA, PC ^{[1
]}

CULLINANE, AA ^{[1
]}

ONIONS, DE ^{[1
]}

NICOLSON, L ^{[1
]}

机构：

[1] IRISH EQUINE CTR, NAAS, KILDARE, IRELAND

来源：

EQUINE VETERINARY JOURNAL | 1992年 / 24卷 / 01期

关键词：

D O I：

10.1111/j.2042-3306.1992.tb02772.x

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The polymerase chain reaction (PCR) is a sensitive technique used to detect DNA of viral pathogens. We have applied the technique to the detection of Equid herpesviruses-1 and -4 (EHV-1 and EHV-4) DNA within nasopharyngeal swab samples from horses. Ninety-eight samples from suspected field cases and in-contact horses were analysed. The assays were conducted blind and later decoded and compared with virus isolation data. Our result indicate that PCR is a sensitive and rapid technique for the diagnosis of EHV-1 and EHV-4 infection.

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页码：20 / 25

页数：6

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