DNA OF MYCOBACTERIUM-TUBERCULOSIS IN FORMALIN-FIXED, PARAFFIN-EMBEDDED TISSUE IN TUBERCULOSIS AND SARCOIDOSIS DETECTED BY POLYMERASE CHAIN-REACTION

被引:110
作者
POPPER, HH [1 ]
WINTER, E [1 ]
HOFLER, G [1 ]
机构
[1] GRAZ UNIV,SCH MED,INST PATHOL,MOLEC PATHOL LABS,GRAZ,AUSTRIA
关键词
MYCOBACTERIA; PARAFFIN-EMBEDDED TISSUE; POLYMERASE CHAIN REACTION; SARCOIDOSIS; TUBERCULOSIS;
D O I
10.1093/ajcp/101.6.738
中图分类号
R36 [病理学];
学科分类号
100104 ;
摘要
Infection with Mycobacterium tuberculosis is a major cause of death worldwide. Identification of mycobacteria in tissue sections is usually easily achieved by acid-fast stains, but this method sometimes gives unsatisfactory results. The authors therefore compared conventional staining techniques and polymerase chain reaction (PCR) for mycobacterial DNA sequences in 24 selected tissue samples from patients with tuberculosis. In all samples, either positive or negative with acid-fast stain, mycobacterial DNA fragments were detected. In addition, tissue samples from patients with clinically proven sarcoidosis were included as controls. Surprisingly, strong signals for mycobacterial DNA were found in 2 of 15 cases. Polymerase chain reaction is a useful technique in the demonstration of mycobacterial DNA fragments in patients with clinically suspected tuberculosis who have acid fast stain-negative histology. An epithelioid granulomatous reaction in the lung, negative by acid-fast stain and positive for mycobacterial DNA by PCR, however, does not permit a diagnosis of tuberculosis, because a positive result can also be obtained in cases of sarcoidosis. In some cases of sarcoidosis, the causal agent might be either cell wall defective mycobacteria or persistent intracellular DNA from mycobacteria.
引用
收藏
页码:738 / 741
页数:4
相关论文
共 22 条
[1]   A SEARCH FOR MYCOBACTERIAL DNA IN GRANULOMATOUS TISSUES FROM PATIENTS WITH SARCOIDOSIS USING THE POLYMERASE CHAIN-REACTION [J].
BOCART, D ;
LECOSSIER, D ;
DELASSENCE, A ;
VALEYRE, D ;
BATTESTI, JP ;
HANCE, AJ .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1992, 145 (05) :1142-1148
[2]  
BURNET FM, 1959, CLONAL SELECTION THE, P160
[3]   AMPLIFICATION OF A SPECIES-SPECIFIC DNA FRAGMENT OF MYCOBACTERIUM-TUBERCULOSIS AND ITS POSSIBLE USE IN DIAGNOSIS [J].
DELPORTILLO, P ;
MURILLO, LA ;
PATARROYO, ME .
JOURNAL OF CLINICAL MICROBIOLOGY, 1991, 29 (10) :2163-2168
[4]   POLYMERASE CHAIN-REACTION AMPLIFICATION OF A REPETITIVE DNA-SEQUENCE SPECIFIC FOR MYCOBACTERIUM-TUBERCULOSIS [J].
EISENACH, KD ;
CAVE, MD ;
BATES, JH ;
CRAWFORD, JT .
JOURNAL OF INFECTIOUS DISEASES, 1990, 161 (05) :977-981
[5]   DETECTION OF MYCOBACTERIUM-TUBERCULOSIS IN SPUTUM SAMPLES USING A POLYMERASE CHAIN-REACTION [J].
EISENACH, KD ;
SIFFORD, MD ;
CAVE, MD ;
BATES, JH ;
CRAWFORD, JT .
AMERICAN REVIEW OF RESPIRATORY DISEASE, 1991, 144 (05) :1160-1163
[6]   GENUS-SPECIFIC AND SPECIES-SPECIFIC DNA PROBES TO IDENTIFY MYCOBACTERIA USING THE POLYMERASE CHAIN-REACTION [J].
FRIES, JWU ;
PATEL, RJ ;
PIESSENS, WF ;
WIRTH, DF .
MOLECULAR AND CELLULAR PROBES, 1990, 4 (02) :87-105
[7]  
Ghossein R A, 1992, Diagn Mol Pathol, V1, P185, DOI 10.1097/00019606-199203000-00027
[8]  
Graham D, 1988, SARCOIDOSIS OTHER GR, P161
[9]  
Hanngren A, 1987, Sarcoidosis, V4, P101
[10]  
MITCHELL DN, 1981, 9TH P INT C SARC, P132