PHENOTYPIC AND GENOTYPIC ANALYSIS OF BORRELIA-BURGDORFERI ISOLATES FROM VARIOUS SOURCES

被引：103

作者：

ADAM, T ^{[1
]}

GASSMANN, GS ^{[1
]}

RASIAH, C ^{[1
]}

GOBEL, UB ^{[1
]}

机构：

[1] UNIV FREIBURG, INST MED MIKROBIOL & HYG, W-7800 FREIBURG, GERMANY

来源：

INFECTION AND IMMUNITY | 1991年 / 59卷 / 08期

关键词：

D O I：

10.1128/IAI.59.8.2579-2585.1991

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A total of 17 B. burgdorferi isolates from various sources were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis of whole-cell proteins, restriction enzyme analysis, Southern hybridization with probes complementary to unique regions of evolutionarily conserved genes (16S rRNA and fla), and direct sequencing of in vitro polymerase chain reaction-amplified fragments of the 16S rRNA gene. Three groups were distinguished on the basis of phenotypic and genotypic traits, the latter traced to the nucleotide sequence level.

引用

页码：2579 / 2585

页数：7

共 62 条

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