BROADLY NEUTRALIZING MONOCLONAL-ANTIBODIES TO THE V3-REGION OF HIV-1 CAN BE ELICITED BY PEPTIDE IMMUNIZATION

被引：100

作者：

WHITESCHARF, ME ^{[1
]}

POTTS, BJ ^{[1
]}

SMITH, LM ^{[1
]}

SOKOLOWSKI, KA ^{[1
]}

RUSCHE, JR ^{[1
]}

SILVER, S ^{[1
]}

机构：

[1] REPLIGEN CORP,CAMBRIDGE,MA 02139

来源：

VIROLOGY | 1993年 / 192卷 / 01期

关键词：

D O I：

10.1006/viro.1993.1022

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The third hypervariable region (V3) of the HIV-1 envelope gp120 protein contains the principal neutralizing domain. Most neutralizing antibodies directed toward this region are very type-specific. Conserved sequences do exist within this region, however, and may prove useful in developing vaccines and therapeutic monoclonal antibodies (MABs) capable of targeting diverse HIV-1 isolates. We have used synthetic peptides containing conserved V3 sequences as immunogens to produce a panel of neutralizing MABs. The characterization of these MABs is described here. In addition, a series of in vitro assays has been developed that may be useful in predicting the neutralization potential of individual antibodies. © 1993 Academic Press. All rights reserved.

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页码：197 / 206

页数：10

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